Basic studies on possible application of anti-oxidative fraction and constituents of crude drug origin to preventive medicine

1.化学研究已被密集地用于提取甲基氯化物（PT ext.）Glycyrrhiza glabra root（俄罗斯律师协会）：四个小的苯甲酸，两个异黄酮。（RL-Q和RL-R）、一个3-芳基香豆素（RL-U）和一个色素瘤（RL-V），所有这些化合物都是未定义的文学，这些化合物被用作2'，4'-二羟基-[6 '，6'-dimethylpyrano（2"，3"：7，8）]-isoflavanone， [6"，6"-dimethylpyrno（2"，3"：7，8）]-2'-hydroxy，4'-methoxy-isoflavanone，4'，7-dihydroxy-[6"，6"-dimethylpyno（2"，3"：2'，3'）]-3-\coumarin [6'，6'-dimethylpyrano（2'，3' ： 7， 8）]-benzopyran-3-ol， respectively， on the spectroscopic basis.2。从俄罗斯许可证中发现的Constituins被描述为经过测试的抗微生物活动，包括MRSA、酵母、moulds和其他。本许可证显示了对微生物活性的广泛多样性的有机化合物.3。上一篇：上一篇：被指控为反主义 ... More - 氧化物的氧化物活性，超氧化物阴离子的清除活性（SOI-D3-（/）2-D3）在两个xanthine-xanthine氧化酶和微系统中产生，并在两个微氧系统中预防脂质过氧化物活性。许多特许权常量在各种测定系统中发现了大量的非氧化物活动，这些证据表明特许权提取（PT ext.）它是一种被认为是在体外基础上预防疾病的承诺剂，可被怀疑在体外基础上被隐含。这是一个特别的兴趣，注意到RL-M展示了经测试的最有潜力的抗氧化物活动，因为它可能不存在的catechoiety，因为它是在自然生长的抗氧化物中找到了非常常见的，也展示了对人类细胞的氧化氢化合物活动，然而，这一调查未能成功地评估净化的宪法的反氧化物组化活动，并将其直接对红细胞膜的作用。Less（低）

1. Chemical investigation was carried out intensively on the methylene chloride extract (PT ext.) of Glycyrrhiza glabra root (Russian licorice) to afford four minor phenolic constituents, two isoflavanones (RL-Q and RL-R), one 3-arylcoumarine (RL-U) and one chromenochromane (RL-V), all of which were undescribed literally, Structures of these compounds were elucidated as 2',4'-dihydroxy-[6",6"-dimethylpyrano(2",3":7,8)]-isoflavanone, [6",6"-dimethylpyrno(2",3":7,8)]-2'-hydroxy,4'-methoxy-isoflavanone, 4',7-dihydroxy-[6",6"-dimethylpyno(2",3":2',3')]-3-arylcoumarin [6',6' -dimethylpyrano(2', 3' : 7, 8)]-benzopyran-3-ol, respectively, on the spectroscopic basis.2. Constituents obtained from Russian licorice described above were tested for anti-microbial activity against gram-positive bacteria including MRSA, yeasts, moulds, and so on. Some of phenolic constituents of this licorice showed potent activity against wide variety of microorganisms.3. The above constituents were assayed for anti … More -oxidative activity on linoleic acid, scavenging activity of superoxide anion (SOィイD3-(/)2ィエD3) generated in both xanthine-xanthine oxidase and microsomal systems, and preventing activity of lipid peroxidation in both microsomal and mitochondrial systems. Many of licorice constituents exhibited outstanding anti-oxidative activity in various assay systems indicating that licorice extract (PT ext.) is a promising agent for the prevention of disease in which active oxygen species are suspected to be implicated on the in-vitro basis. It is of particular interest to note that RL-M showed the most potent anti-oxidative activity among licorice constituents tested though it does not possess catechol moiety that is fond very commonly in naturally occurring antioxidants, Licorice extract also showed significant inhibitory activity against oxidative hemolysis of human erythrocytes, but this assay failed to assess anti-oxidative hemolysis activity of purified constituents due to their direct action to erythrocyte membrane. Less