Analysis of Carrier-Mediated Transport Systems in Drug Absorption from Oral Mucosa

该体外进行的研究是利用人口腔穆科萨、布卡和舌头的分离细胞进行实验，以研究葡萄糖和寡肽进行实验的机理。D-葡萄糖的升级在细胞中的舌头和比在Buccal细胞中的多糖大得多，并且被2-deoxy-D-葡萄糖，一个facilitative glucose transporters的基础，比α-甲基-D-葡萄糖side的基础，一个特定的基础，建议更大的facilitative transporters的贡献比Na-D1+ Yie D1/葡萄糖转运器。Furthermore，根据Oral Mucosa对抑制性研究的结果，包括麦芽糖和D-甘露糖、GLUT 1和/或GLUT 3建议将葡萄糖上调的部分纳入葡萄糖。因此，我们试图确认葡萄糖转运器在口腔mucosa上的表达，用西方blotting。作为一个结果，它被认为SGLT 1、GLUT 1、GLUT 2和GLUT 3被描述为人类口腔穆科萨的基本细胞。关于glycylsarcosine、一种dipeptide模型的研究的结果，由孤立的口腔细胞展示了载体介导的传输系统在人类口腔mucosal细胞中寡肽的存在。通过文化人类口腔粘液细胞的结构化细胞层的D-葡萄糖和糖基酶的传输也进行了测试，并显示了该传输器功能不仅适用于通过这些粘液细胞层的迁移，但也适用于通过结构化粘液细胞层的传输。

The in vitro uptake study was performed using the isolated cells of human oral mucosa, buccal and the dorsum of tongue, to investigate the mechanisms of glucose and oligopeptide uptake. The uptake of D-glucose was much larger in cells of the dorsum of tongue than in buccal cells and was inhibited more extensively by 2-deoxy-D-glucose, a substrate of facilitative glucose transporters, than by α-methyl-D-glucoside, a specific substrate of SGLT1, suggesting the larger contribution of facilitative transporter than NaィイD1+ィエD1/glucose cotransporter. Furthermore, from the results of inhibition studies by the several sugar analogues including maltose and D-mannose, GLUT1 and/or GLUT3 were suggested to take part in the glucose uptake by oral mucosa. Therefore, we have attempted to confirm the expression of glucose transporters on the oral mucosa by employing Western blotting. As a result, it was suggested that SGLT1, GLUT1, GLUT2 and GLUT3 are expressed in the epithelial cells of human oral mucosa. The result of the uptake study of glycylsarcosine, a model dipeptide, by isolated oral cells showed the presence of carrier-mediated transport systems for oligopeptides in human oral mucosal cells. The transport of D-glucose and glycylsarcosine across stratified cell layer of cultured human oral mucosal cells was also examined, and the results showed that the transporters function not only for the uptake of these substrates by the mucosal cells but also for the transport across the stratified mucosal cell layers.