Premier GC/Q-TOF with headspace and SPME analysis

Ischebeck实验室的主要研究课题是研究脂滴、含有疏水化合物的亚细胞结构和嵌入周围磷脂单层的蛋白质。研究脂滴在代谢（尤其是固醇代谢）中的作用，以及对功能未知的脂滴相关酶进行生化分析，需要灵敏的分析工具。将脂滴转化为萜类化合物工厂和储存库的生物技术方法也需要能够测量前体和产物。此外，Ischebeck实验室在合作项目方面有着良好的记录，在这些项目中，它为植物、微生物和神经科学领域的小组提供了基于气相色谱-质谱的分析。最近，Ischebeck实验室转移到了世界自然大学<s:1>国家科学技术研究所，这也激发了该学院对这些分析的兴趣。Ischebeck实验室在GC-MS分析方面积累了丰富的经验，但自从小组转移到WWU以来，研究所或地点没有最先进的气相色谱-质谱联用系统。然而，实验室的项目和协作工作需要直接访问这样的仪器。气相色谱-四极杆飞行时间-质谱联用仪可在靶向和非靶向方法中提供高灵敏度的疏水物质分析。准确测定代谢物及其片段的分子质量有助于鉴定物质。通过添加顶空和固相微萃取模块，挥发物的分析得到了极大的增强。通过衍生化，还可以鉴定和绝对定量极性物质，包括糖、氨基酸和其他中心代谢物。总之，该仪器将提供大量化合物的高灵敏度和定量测量，极大地增加了实验室和生物学教师的研究可能性。

The main research topic of the Ischebeck lab is the study of lipid droplets, subcellular structures that contain hydrophobic compounds and proteins embedded in the surrounding phospholipid monolayer. The study of the role of lipid droplets in metabolism (especially sterol metabolism), and the biochemical analysis of lipid droplet-associated enzymes of unknown function requires sensitive analytical tools. Biotechnological approaches to turn lipid droplets into factories and storage depots for terpenoids also needs possibilities to measure precursors and products. Furthermore, the Ischebeck lab has a strong track record of collaborative projects, where it provided gas chromatography-mass spectrometry-based analytics to groups of the plant, microbiology and neuroscience field. The recent transfer of the Ischebeck lab to the WWU Münster also sparked interest in these analytics for several projects at the faculty. The Ischebeck lab collected a lot of experience in GC-MS analytics but since the transfer of the group to the WWU, no state-of-the art gas chromatograph-mass spectrometer system is available at the institute or the location. However, a direct access to such an instrument is required for the projects of the lab and the collaborative efforts. The gas chromatograph-quadrupole-time of flight-mass spectrometer would provide highly sensitive analytics of hydrophobic substances both in targeted and untargeted approaches. The exact determination of the molecular mass of metabolites and their fragments would help to identify substances. By the addition of the headspace and solid-phase-microextraction modules, analysis of volatiles is strongly enhanced. Through derivatization, it is also possible to identify and absolutely quantify polar substances including sugars, amino acids, and other central metabolites. In conclusion, the instrument would provide highly sensitive and quantitative measurements of a large variety of compounds, strongly increasing the research possibilities of the lab and the faculty of biology.