Development of selective, rapid and simple sensing system for oraganohalogen Compound

最近，由于二恶英和双酚A等内分泌干扰物造成的环境污染在日本成为一个严重的问题。然而，由于缺乏简便、快速的测定内分泌干扰物的分析方法，对野外环境污染的真实状况没有进行充分的研究。内分泌干扰物的测定通常采用气相色谱-质谱联用（GC-MS）法。气相色谱-质谱法测定内分泌干扰物耗时长，技术要求高。因此，对环境领域中检测和测量内分泌干扰物的便携、简便、快速的分析方法的需求日益迫切。本课题利用线阵CCD对传感器的反射光进行检测，研制了一种便携式、手持式SPR传感器。研制的SRR传感器尺寸约为8厘米× 6厘米× 4.8厘米。传感器重量小于1kg。处理步骤采用以邻苯二甲酸二丁酯和磷酸三甲酯为原料，以1:1的质量比制备增塑型PVC薄膜，建立了棱镜与传感器芯片之间的新型光界面。所得PVC薄膜的折射率为1.561。该薄膜具有与传统配合油相同的折射率。研制了一种用于便携式SPR传感器的传感芯片。传感器芯片为2cm高的圆柱形试管，试管末端在玻璃上附着一层薄薄的金膜。匹配膜对传感器芯片具有很强的粘性，适合贴附在便携式SPR传感器上。所研制的SPR传感器成功地用于测定0.1 ppm水平的2,4-二氯苯酚

Recently, environmental pollution due to endocrine disruptors such as dioxin and bis-phenol A is becoming a serious problem in Japan. However, the real state of the environmental pollution in field is not examined adequately because no simple and rapid analytical methods for the determination of endocrine disruptors were reported. The determination of endocrine disruptors is usually determined by gas chromatography-mass spectrometry (GC-MS). The procedure of GC-MS for the determination of endocrine disruptors is time-consuming and needs a very skilled technique. As a result, urgent needs have been developed for potable, simple and rapid analytical methods for the detection and measurement of endocrine disruptors in environmental field. In this project, a portable and handy-type SPR sensor was developed by using a line CCD for detection of reflected light of the sensor. The dimension of the developed SRR sensor was about 8 cm x 6 cm x 4.8 cm. The weight of the sensor was less than 1 kg. A new light interface between the prism and the sensor chip was developed by using a plasticized PVC film, which was prepared from dibutylphthalate and tricrediyl phosphate at the weight ratio of 1 : 1. The refractive index of the prepared PVC film was 1.561. This film has the same refractive index as of the conventional mating oil. A sensor chip for the portable SPR sensor was newly developed. The sensor chips was cylidrical cuvette with 2 cm-hight, and a thin gold film on the glass was attached at the end of the cuvette. The matching film, which was very sticky to the sensor chip, was suitable for attaching on the portable SPR sensor. The developed SPR sensor was successfully applied to the determination of 2,4-dichlorophenol down to 0.1 ppm level