Development ofhighly sensitive influenza virus sensor base on Pressure-light conversion element

基于压光转换元件的高灵敏流感病毒传感器的研制

• 批准号: 22550076
• 负责人: IMATO Toshihiko
• 金额: $ 3.08万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22550076/
• 关键词: 圧力センサ; イムノアッセイ; 水晶振動子; インフルエンザ; 免疫測定; 磁気ビーズ; 光学的検出; 圧力-光変換素子

An immunoassay technique has been widely used for many analytical fields such as diagnosis of influenza virus as well as environmental assessment and safety of foods. In such cases, immunoreactions with an antigenand an antibody have been utilizedfor highly selective analysis. However since there need many tedious and time-consuming protocols, a more rapid and simpler analytical technique is desired. In addition, in some cases, a method for labeling an enzyme to an antibody or an antigen is used for enhancement of the sensitivity of an immunoassay, but a much sensitive analytical technique would be expected. Inthis work, in order to enhance the rapidity of analysis and the sensitivity of the immunoassay, we tried to use magnetic microbeads for a support of the immunoassay and to enhance the analytical signals by using an element of piezoelectric device for detection of magnetic microbeads by applying outer magnetic fields. We have developed an immobilization method of an antibody to the microbeads and clarified the relationship between the strength of the magnetic fields and the signal of piezoelectric device. As a result a new immunoassay method has been proposed by using the present technique as the model analyte of immunoglobulin.

免疫分析技术已广泛应用于许多分析领域，如流感病毒的诊断以及环境评估和食品安全。在这种情况下，抗原和抗体的免疫反应被用于高选择性分析。然而，由于需要许多繁琐和耗时的协议，需要一个更快速和更简单的分析技术。此外，在某些情况下，将酶标记到抗体或抗原上的方法用于增强免疫测定的灵敏度，但是将期望更灵敏的分析技术。本工作中，为了提高分析的快速性和免疫分析的灵敏度，我们尝试用磁性微球作为免疫分析的载体，并利用压电元件对磁性微球进行检测，通过施加外磁场来增强分析信号。我们发展了一种抗体固定于微珠的方法，并阐明了磁场强度与压电器件信号之间的关系。结果表明，该技术可作为免疫球蛋白的模型分析物，建立一种新的免疫分析方法。

项目成果

Development of a novel two dimensional surface plasmon resonance sensor using multiplied beam splitting optics.

• DOI: 10.3390/s130100801
• 发表时间: 2013-01-08
• 期刊: Sensors (Basel, Switzerland)
• 作者: Hemmi A;Mizumura R;Kawanishi R;Nakajima H;Zeng H;Uchiyama K;Kaneki N;Imato T
• 通讯作者: Imato T

Performance of a light emitting diode with spectrally narrow emission based on waveguide mode and application to a light source of flow-fluorometry on microchip

基于波导模式的窄光谱发光二极管的性能及其在微芯片流式荧光测定光源中的应用

• 发表时间: 2012
• 作者: T. Takayanagi;K. Hojyo;F. Iwami;S. Motomizu;工藤義広;Toshihiko Imato
• 通讯作者: Toshihiko Imato

Fluorometric Flow Immunoassay for IgA by using Microchip Integrated with Organic Thin Film Photodiode

使用有机薄膜光电二极管集成微芯片进行 IgA 荧光流式免疫测定

• 发表时间: 2011
• 作者: M. Miyake;N. Soh;K. Nakano;M. Yahiro;C. Adachi;A. Hemmi;H. Nakajima;K. Uchiyama;T.Imato
• 通讯作者: T.Imato

A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore.

• DOI: 10.1016/j.talanta.2012.02.025
• 发表时间: 2012-05
• 期刊: Talanta
• 影响因子: 6.1
• 作者: Daisuke Seto;T. Maki;N. Soh;K. Nakano;R. Ishimatsu;T. Imato

"Organic Photo Diode as a Detector of Fluorescence Immunoassay-Flow Injection Analysis of Alkylphenol Ethoxylate on a Microchip-

“有机光电二极管作为微芯片上烷基酚乙氧基化物的荧光免疫测定-流注射分析检测器-

• 发表时间: 2012
• 作者: A. Naruse;○R. Ishimatsu;K. Nakano;M. Yahiro;C. Adachi;T. Imato
• 通讯作者: T. Imato