Effects of Dietary Fiber on Digestive/Absorption Function Assessed by the Ileum Contents Perfusion Method

本研究采用回肠内容物灌注法，研究膳食纤维对人体消化吸收功能的影响。1. 测量回肠末端的膳食纤维（抗性淀粉和果胶）的价值，以评估这些纤维作为膳食纤维的真实价值。2. 为了评估大肠发酵的热量摄入，测量了膳食纤维（抗性淀粉、果胶、乳果糖和纤维素）摄入后的呼出气体。3. 膳食纤维对外源性中性脂肪吸收的影响。结果表明，回肠末端收集的果胶平均含量为85.0±10.8%。10g抗性淀粉的平均转化率为34.7±9.7%。在两项研究中，个体之间的差异为20%。因此，估计果胶和抗性淀粉作为膳食纤维的价值分别为90%和35%左右。在大肠纤维发酵方面，膳食纤维的发酵模式在不同个体和不同种类的膳食纤维之间存在差异。至于纤维对中性脂肪吸收的影响，单次给予5g果胶不抑制外源脂质的吸收。因此，为了在临床环境中降低患者的胆固醇和甘油三酯水平，因此认为有必要每天长期服用5g果胶作为DF补充剂。我们可以明确膳食纤维在人体中的代谢部分及其对消化吸收功能的影响，我们相信我们的研究结果将促进未来膳食纤维研究的进展。

In this study, the effects of dietary fiber on digestive/absorption function in human were studied using Ileum Contents Perfusion Method as follows. 1. Measured the values of dietary fiber (resistant starch and pectin) in the terminal ileum to evaluate the true values of these fibers as dietary fiber. 2. In order to evaluate the calorie ingestion by the fermentation in the large intestine, the exhaled gas after dietary fiber (resistant starch, pectin, lactulose and cellulose) ingestion were measured. 3. Effect of dietary fiber on absorption of exogenous neutral fat using breath test. As a result, the mean rate of 5g of pectin collected at the terminal ileum was calculated as 85.0±10.8%. However, the mean rate of 10g of resistant starch was calculated as 34.7±9.7%. Differences among individuals were 20% in both studies. Therefore, the values of pectin or resistant starch as dietary fiber were estimated at about 90% or 35%, respectively. As for fermentation of fiber in the large intestine, patterns of fermentation of dietary fiber were differences among individuals and types of dietary fiber. As for effects of fiber on absorption of neutral fat, a single administration of 5g of pectin did not inhibit the absorption of exogenous lipids. Therefore, in order to reduce the cholesterol and triglyceride levels of patients in a clinical setting, a much longer-term daily administration of 5g of pectin as a DF supplement is thus considered necessary. We can clarify the part of metabolism of dietary fiber and its effects on digestive/absorption function in human body, and we believe that our results stimulate the progress of dietary fiber research in the future.