THE ANALYSIS OF PIG GENE CLUSTER EXPRESSION IN THE LUNG CANCER

肺癌是一种预后较差的疾病，对治疗表现出耐药性。p53诱导基因（PIG）表达的改变是导致这种顽固性疾病的原因之一。本研究以阐明PIG基因簇的表达变化并在一开始给予肺癌基因治疗进一步发展为目的。经支气管镜或手术采集的肺癌组织，在10%FCS RPMI中，用CDDP、BLM、紫杉醇等抗癌药物培养一段时间。并检测p53基因簇是否激活。通过腺病毒将p53引入患者肺癌的基因治疗于去年12月在我院开始。同时制作患者的描述和协议文件。该临床研究开始时，也获得了伦理委员会的许可。迄今为止，本院仅有少数患者证实p53基因异常。这比此后报告的频率（约50%）要低。51岁男性肺癌患者给予CDDP和Ad5CMV-p53治疗。但他拒绝接受任何进一步的抗癌治疗，因此无法对其减瘤效果进行正式评价。但观察到暂时的肿瘤缩小效果，他的运动状态没有改变。在猪基因的分析上，必须包括许多患者。今后，我们将进一步努力。

The lung cancer is a disease of poor prognosis, which shows the resistance for the treatment. The change of expression of p53 induced gene (PIG) as one of the causes of this refractory. In this study, it is made that lung cancer gene therapy that it clarified the change of the expression of the PIG gene cluster and gave in the beginning develops further to be a purpose. The tissue of the lung cancer collected via bronchoscope or operatively is cultured in 10%FCS RPMI with anti-cancer drugs such as CDDP, BLM and Pacritaxel for some periods. And examine whether p53 gene cluster is activated or not. The gene therapy, which introduced p53 into lung cancer of the patient by Adeno virus, was started in December, last year in our hospital. Description and agreement document to the patient were also being made. When this clinical research was started, the permission of the ethics committee was also obtained. Only few patients were proved the p53 gene abnormality in our institute until now. It is less than the frequency by the report ever since (about 50%). 51-year old man who suffered with lung carcinoma was given CDDP and Ad5CMV-p53. But he refused further any anti-cancer treatment, therefore the formal evaluation about tumor reduction effect was impossible. But the temporary tumor reduction effect was observed and his performance status did not change.On the analysis of the PIG gene, many patients must be included. In future, we will do further effort.