Effect to angiogenesis due to implantation of autologous tissue and fibroblasts in ischemic heart

从体外，兔耳室(REC)提供了一种独特的系统来观察体内的毛细血管。大多数房间是由透明的易反射材料制成的，包括玻璃、丙烯酸树脂和云母。REC中毛细血管的宏观图像质量并不令人满意。如果后面有照明灯，照明弹就很常见，而闪光灯就会反射。为了获得清晰的、对比度好、边缘清晰、无耀斑的REC宏观图像，我们制作了一套新的系统。我们在尼康多功能照相架上使用了尼康F4s和医用Nikkor。在一个黑色的灯箱里，装了一个闪光灯、尼康微距照明灯SB-21和一个模型灯。在灯箱顶部的边缘打了一个直径1厘米的洞。拍摄工作进行得很顺利。在没有麻醉或给动物施加压力的情况下，拍摄了一两分钟的照片。未经治疗，REC区在第7天观察到血管生成，第4周血管形成完全。术后6个月内图像质量良好，对比度好，边缘清晰，无光晕。我们的结果表明，该系统很好地获得了体内血管生成的直接证据，在很长一段时间内，每周间隔一次。

From outside the body, the rabbit ear chamber (REC) provides a unique system for viewing capillaries in vivo. Most chambers are made of transparent easy-reflect materials including glass, acrylic resin and mica. The quality of macroscopic pictures of capillaries in the REC has not been satisfactory. With an illuminator from behind, flare was common, and with a flash light, reflection occurred. To obtain clear macroscopic pictures of REC with good contrast and sharp edges without flare, we made a new system. We used a Nikon F4s with a Medical Nikkor on a Nikon Multi photo stand. In a black light box, a strobe, Nikon Macro Speed Light SB-21, and a Modeling lamp were loaded. A 1-cm diameter hole was made at the edge on the top of the light box. Filming was done without difficulty. Without anesthetizing or stressing the animal, pictures were taken for one or two minutes. Without treatment, angiogenesis was observed at day 7 and vasculature was complete at 4 weeks in the REC field. The quality of pictures was satisfactory with contrast and sharp edges without flare up to 6 months. Our results suggest that this system is excellent to obtain direct evidence of angiogenesis in vivo from immediately after at weekly intervals over a long period.