Clinical application of repetitive transcranial magnetic stimulation for motor functional reconstruction

本研究观察了高频磁刺激对小鼠中枢神经系统即刻早期基因（c-fos）、神经保护基因（NGF或BDNF）和胶质细胞酸性蛋白（GFAP）表达的影响。高频经颅磁刺激（1-30串，25 Hz，持续时间10秒）通过4 cm直径的圆形线圈经颅递送至小鼠。刺激产生刺激锁定运动反应，但没有引起行为癫痫发作。用原位杂交法检测刺激后12、24、36、48、96、192小时BDNF、c-fos和GFAP的表达。在多次磁刺激（25 Hz，10秒）后，尽管大脑的广泛区域被激活，但在齿状回中GFAP的mRNA水平增加了几倍。GFAP mRNA表达上调的模式与电惊厥发作（ECS）和中枢神经系统损伤后相似。应用rTMS对脊髓损伤模型进行治疗可调节损伤后的基因表达和行为活动（Tarlov评分2-3），这些结果表明，侵入性磁刺激对神经元和神经胶质细胞基因表达具有强有力的影响，基因表达和神经重组。

The present study evaluates the consequences of high frequency magnetic stimulation on the expression of immediate early gene (c-fos), neuroprotective genes (NGF or BDNF) and glial fibrillary acidic protein (GFAP) by in the murine CNS. High frequency transcranial magnetic stimulation (1-30 trains at 25 Hz of 10 sec duration) was delivered transcranially to mice via 4 cm diameter round coils. The stimulation produced stimulus-locked motor responses but did not elicit behavioral seizures. BDNF, c-fos and GFAP levels were evaluated 12, 24, 36, 48, 96, 192 hrs following stimulation by in situ hybridization. Following multipe trains of magnetic stimulation (25 Hz for 10 sec.), the levels of mRNA for GFAP increased several fold in the dentate gyrus despite the fact that widespread areas of the brain were activated. The pattern of GFAP mRNA upregulation was similar to that obtained following electroconvulsive seizures (ECS) and central nervous system injury.Application of rTMS to spinal cord injury model modulated gene expression following injury and behavioral activity (Tarlov score 2-3) followed electrophysiological finding.These results indicate that non-invasive magnetic stimulation has a powerful influence on neuronal and gliai cell gene expression inducing changes that are similar to the changes that occur following nervous system injury so that modulate gene expression and neural reorganization.