Effect of Growth Factors on Cultured Human Hypertrophic Scar Fibroblasts

关于伤口修复，在增生性瘢痕和瘢痕疙瘩的形成中，有重要但未知的因素参与成纤维细胞功能的调节。本研究旨在探讨肿瘤坏死因子-α（TNF -α）和转化生长因子-β（TGF-β）两种生长因子对瘢痕疙瘩成纤维细胞胶原酶活性的影响。在暴露于TNF - α后，在人瘢痕疙瘩成纤维细胞中发现胶原酶活性水平增加。在TNF - α和TGF- β暴露后，发现人瘢痕疙瘩成纤维细胞中胶原酶活性水平降低。这些结果表明，TGF- β可以抑制TNF - α刺激的人瘢痕疙瘩成纤维细胞的胶原酶活性，表明生长因子和细胞因子可能在瘢痕疙瘩形成中起治疗作用。

Regarding wound repair, there are important but as yet unknown factors involved in the regulation of fibroblast function in the formation of hypertrophic scars and keloids. If these factors could be clarified, new therapies for keloids could be developed.Recently, some researchers have reported that growth factors and cytokines possess some abilities to control hypertrophic scar and keloid formation.The purpose of this study was to investigate whether the collagenase activity in human keloid fibroblasts is stimulated by two kinds of growth factors, TNF -α and TGF-β. Increased levels of collagenase activity were found in human keloid fibroblasts following exposure to TNF - α. A decreased level of collagenase activity was found in human keloid fibroblasts following exposure to both TNF - α and TGF- β. These results indicate that TGF- β can inhibit collagenase activity in human keloid fibroblasts stimulated by TNF - α, suggesting that growth factors and cytokines may play a therapeutic role in keloid formation.