Periosteal regeneration and bone augmentation using recombinant human BMP-2/atelocollagen onlay implant

在重组人骨形态发生蛋白-2骨膜下植入系统中评价皮质穿孔有限暴露骨髓的效果。将10μg重组人骨形态发生蛋白-2溶液和10 mg胃蛋白酶消化的胶原(Atelocollagen)溶液混合在灭菌管中冷冻干燥。将重组人骨形态发生蛋白-2(10μg)/胶原(10 Mg)复合体植入10月龄Wistar大鼠的骨膜与颅骨间隙内。分别于术后1、2、3、4周取出种植体和颅骨，制作厚度为4μm的脱钙冠状切片，苏木精-伊红-弹力-范吉森染色。有皮质穿孔的rhBMP-2/胶原植入后1周和2周时，成骨速度开始加快，而未穿孔的rhBMP-2/胶原植入后1周和2周，周围骨中部有大量圆形空隙。此外，在4周时，rhBMP-2/胶原植入物完全被新骨取代。我们目前的结果表明，有限的皮质植入宿主床有助于促进早期成骨，这种新型的rhBMP-2/atelocollagen植入系统，作为一种可吸收的装置，被新骨取代，将带来更好的和更可预测的骨强化治疗。

The efficacy of limited exposure of bone marrow by cortical perforations were evaluated in subperiosteal rhBMP-2 implant system. Ten μg of rhBMP-2 solution and 10 mg of pepsindigested collagen (atelocollagen) solution were mixed in the sterilized tube and lyophilized. The composition of rhBMP-2 (10 μg)/collagen (10 mg) was implanted into the space between the periosteum and skull with or without outer cortical perforations in 10-month old Wistar rats. At 1, 2, 3 and 4 weeks after operation, the implant and the skull were removed en bloc and decalcified coronal sections (4 μm in thickness) were made and stained with hematoxylin and eosin and Elastica van Gieson. RhBMP-2/collagen implant with cortical perforations resulted in an initial acceleration of osteogenesis, whereas the rhBMP-2/collagen without perforations resulted in peripheral bone with many round empty spaces in the middle area at 1 and 2 weeks. In addition, the rhBMP-2/collagen implant was completely replaced by newly formed bone at 4 weeks. Our present results indicate that limited cortical petforations to host bed contributed to promote osteogenesis in initial stages and this novel rhBMP-2/atelocollagen implant system, an absorbable device replaced by new bone, would lead to improved and more predictable treatments for bone augmentation.