Application of Optical Biopsy by Fluorescent Endoscopic Observation using a New Photosensitizer

利用5-氨基乙酰丙酸=5- ala在体内产生的光敏剂原卟啉- ix （Pp-IX）对移植于C3H/He小鼠背部的鳞状细胞癌（SCC）肿瘤具有亲和力的新型半导体激光器（iLS-LD400模型，405 nm, 5 mW）的光动力学大诊断（PDMD）进行了研究。结果发现，光谱学数据表明，经47 kHz、50 W、10 min超声照射后，组织中Pp-IX的荧光强度增强2倍。并对临床糖母细胞瘤和星形细胞瘤组织样品进行冷冻和10 μm冷冻切片，研究了Pp-IX的荧光光谱分析。在距离激发光谱405 nm处出现Soret vand峰。新型半导体激光器(iLS-LD400型号，IHI。（405 nm, 5 mW）与Soret vand峰相同。对合并到肿瘤中的Pp-IX进行宏观诊断是非常方便的。此外，从这些样品的激发光谱中还发现，最适合肿瘤手术后光动力学治疗的波长为572- 606,630和670 nm。综上所述，将建立一种新型半导体激光器（iLS-LD400模型，IHI）对临床脑肿瘤组织中含有5-ALA的肿瘤进行内镜诊断，并在570nm更长的波长下使用该激光器治疗脑和/或其他上皮肿瘤。

We investigated the photodynamic macrotic diagnosis (PDMD) of a cancer by a new semiconductor laser (iLS-LD400 model, 405 nm, 5 mW) using the photosensitizer protoporphyrin-IX (Pp-IX) generated in vivo from 5-aminolevulinic acid=5-ALA which has an affinity for the squamous cell carcinoma (SCC) tumor transplanted on the back of C3H/He mouse. In the result, it was found the specific affinity of the Pp-IX in the SCC tumor from the spectroscopic data that the Pp-IX fluorescent intensity in the tissue was enhanced 2 times by the ultrasound irradiation (47 kHz, 50 W, 10 min).Furthermore, it was also investigated the fluorescent spectroscopic analysis of the Pp-IX incorporated in the clinical glyoblastoma and astrocytoma tissue sample which were frozen and were cryo-sectioned at 10 μm. It was found that the Soret vand peak was presented at 405 nm from the excitation spectra. The new semiconductor laser light (iLS-LD400 model, IHI., 405 nm, 5 mW) was same as that of the Soret vand peak. It is very convenient to do a macrotic diagnosis to detect the Pp-IX incorporated into the tumor. Furthermore, it was also found that the best suited wavelengths were 572-606, 630 and 670 nm to treat the tumor photodynamically after the cancer operation from the excitation spectra of these samples.In conclusion, it will be established to do the endoscopic diagnosis for the tumor incorporated 5-ALA in the Clinical brain tumor tissue using a new semiconductor laser (iLS-LD400 model, IHI) and to treat the brain and/or the other epithelium tumors by the lamp at more longer wavelength from 570 nm.