Effect of laser light irradiation on channel characteristic of immunocytologic membrane.

在本研究中，在低功率激光照射（波长：594和780 nm）下，研究了作为巨噬细胞前体细胞的人单核细胞的活性。使用由单核细胞产生的自由基氧测量活性。结果表明，780 nm波长的光照射比594 nm波长的光照射对荧光强度的增强作用更强。在780 nm处，在5-8 J/cm ^2能量范围内有一个活度峰值，超过该能量范围后，活度有降低的趋势。结果表明，不同波长或能量的激光照射红细胞膜，单核细胞的活性增强，同时研究了594和670 nm波长不同能量的激光照射红细胞膜时，红细胞膜通道的特性。使用单通道方法评估所施加的电压和通过膜的电流之间的关系。 ...更多信息 路径夹系统。结果表明，在0 ~ 100 mJ/ cm^2范围内，在670 nm红光照射下，电流与外加电压成正比增加，负电压下电流增加趋势不大。膜的电阻随电压的降低而降低，激光照射使膜的电阻增大。波长为594 nm的黄色激光照射的图案与红色激光照射的图案相似。然而，在35 - 105 mJ/cm ^2的能量范围内，电流的模式发生了逆转，在能量范围之外，电流模式又恢复到对照状态。膜的电阻在所有外加电压范围内均为负值。结果表明，在该能量范围内，细胞膜受到刺激，细胞膜发生去极化。可以存在取决于光的波长或能量而改变的去偏振的阈值水平。少

In this study, the activity of the human monocytes, which are the precursor cells of macrophages, was investigated under the low power laser light irradiation (wavelength : 594 and 780 nm). The activity was measured using the radical oxygen generated from the monocytes. In the results, it was more enhanced by the irradiation of the wavelength of 780 nm than that of 594 nm. At 780nm, there were the peak of the activity at the range of 5-8 J/cm^2 and the tendency toward the low level of the activity beyond the energy range. It suggested that the activity of monocytes was enhanced depending with the wavelengths or energy levels of the laser light.On the other hand, the characteristics of the channel was investigated when the laser light irradiated on the erythrocytologic membrane at the different wavelength of 594 and 670 nm, and the different light energy. The relationships between the applied voltage and the current through the membrane was evaluated using the mono-channel method of the … More path-clamp system. In the results, the current increased linearly in proportion to the applied voltage under the red laser light irradiation of 670 nm in the range of 0 - 100 mJ/ cm^2, and there was an little increasing tendency the current at minus voltages. The resistance of the membrane was decreased in proportion to the decreasing of the voltage, and it was enhanced by the laser light irradiation. The pattern of the yellow laser light irradiation of the wavelength of 594 nm was similar to that of the red laser light irradiation. There was, however, a significant difference which was the reversed pattern of the current in the energy range of 35 - 105 mJ/ cm^2, and the pattern returned to the control state beyond the energy range. The resistance of the membrane show the minus value in all range of the applied voltages. It suggested that the membrane was stimulated and the depolarization occurred on the membrane of the cell at the energy range. There may be the threshold level of the depolarization to be changed in the depending to the wavelength or energy of the light. Less