Project study of tissue regeneration with tissue engineering

1.间充质干细胞：牙周骨缺损处的增殖组织促进牙根周围新牙周组织的形成(山田太田)。我们发现细胞外基质是人牙龈成纤维细胞(Maeda)碱性磷酸酶的诱导和多层结构形成的关键调节因子。经鉴定为COX装配因子的克隆PDL-29在体外培养的HPE中的m RNA表达明显强于HGFs。(Takashiba)2.信号分子：将PGS放置在根表面和rhBMO-2 3PGS复合体之间具有减少强直(Kawanami)的作用。SPARC通过促进增殖和破骨细胞生成抑制因子的产生(栗原)在牙周组织的修复中发挥作用。Emdogain具有促进牙骨质再生和创造良好环境促进牙周再生的潜力。磷酸钙骨水泥似乎是牙周炎…骨形成和组织相容性愈合的支架更多的起诉(Oda)。CAMP升高剂PGE2、EP2/EP4激动剂可刺激HPDL细胞内cAMP积聚。但BMP-2对其无影响，BMP-2预处理对PGE_2或EP2/EP4激动剂(长谷川)刺激的cAMP积聚也无明显影响。LJE变短，而再生结缔组织的增殖活性保持不变，最终LJE被再生结缔组织取代(Hashimoto 9)。动物实验中，局部应用0.1-0.4%的bFGF可显著诱导牙周组织再生。体外实验表明，含胎牛血清的碱性成纤维细胞生长因子可抑制牙周上皮细胞的增殖，并诱导牙周韧带细胞释放透明质酸和硫酸肝素。3.支架材料：与PLGA膜相比，PLLA膜具有更好的体外培养效果。此外，组织学观察显示，PLLA和PLGA膜(Izumi)之间的骨再生没有差异。研究基质几何结构对骨形态发生蛋白诱导牙周重建的影响。这项使用几何不同细胞底物的研究表明，具有一定几何尺寸的基质最有利于细胞分化((Takida)较少

1. Mesenchymal stem cells : Proliferating tissue in periodontal osseous defects promotes the formation of new periodontal tissue around the root(Yamada.Ota). We find that the extracellular matrix is a critical regulator of the induction of alkaline phosphatase and the formastion of multiple layered in human gingival fibroblast(Maeda). One clone, PDL-29, identified as a COX assembly factor, showed much stronger m RNA expression in HPEs than in HGFs in culture. (Takashiba)2. Signaling molecules :Placement of PGS between the root surface and rhBMO-2 3 PGS complex had the effect to decrease ankylosis(Kawanami). SPARC plays arole in repair of periodontal tissues by promotingproliferation and osteoclastogenesis inhibitory factor production(Kurihara). EMDOGAIN has a potential to promote the cementum regeneration and to create a favorable environment that will promote the periodontal regeneration. CPC seemed to act as a scaffold for bone formation and histocompatible healing of periodontal tis … More sues(Oda). PGE2, cAMP-elevating agent, EP2/EP4 agonist stimulated cAMP accumulation in HPDL cells. However, BMP-2 hand no effect on it, and per-treatment with BMP-2 also did not cause significant changes in the cAMP accumulation stimulated with PGE2 or EP2 / EP4 agonist(Hasegawa). LJE becomes shorter, whereas the proliferative activity of regenerative connective tissue maintains the same level of proliferation, and ultimately LJE is replaced by regenerative connective tissue(Hashimoto9.Topical application of 0.1-0.4% bFGF induced significant periodontal tissue regeneration in animal experiments. In vitro studies demonstrated that bFGF stimulation in the presence of fetal calf serum inhibited proliferation of gingival epithelial cells and induced the release of hyaluronan and heparan sulfate from periodontal ligament cells (Murakami)3. Scaffolds : The PLLA membrane showed an excellent results in comparison with the PLGA membrane in vitro experiment. Moreover, the histological observation showed no different bone regeneration in dogs in between PLLA and PLGA membrane(Izumi). The effect of matrix geometry upon the periodontal reconstruction induced by BMP was studied. This study using geometrically different cell substrata demonstrated that a matrix with a certain geometrical size is most favorable for cell differentiation((Takida) Less