The expression analysis of symbiotic nitrogen-carbon

我们对各种豆科植物的尿酸酶基因进行了克隆和测序，并绘制了系统发育树。对不同豆科植物尿酸酶基因序列的系统发育分析表明，转运酰胺和脲的豆科植物的尿酸酶形成了两个不同的类群，表明这两类豆科植物是进化分化的。在纯化了两种尿酸酶后，我们研究了这些尿酸酶的性质。它们对氧的Km值没有不同。然而，一种尿酸酶(UR9)对尿酸的Km值低于另一种尿酸酶(UR2)。功能获得和功能丧失实验表明，大豆尿酸酶基因5‘非编码区的6碱基(GTAATG)基序是结瘤非侵染细胞特异性表达的顺式元件。

We have cloned and sequenced uricase genes of various legume plants and made a phylogenetic tree. As a result, phylogenetic analysis of uricase sequences from various legumes indicated that uricases of amide- and ureide- transporting legumes forms two distinct groups, suggesting these types of legumes were evolutionary divided.The two uricase genes of soybean were expressed at high level using pET vector. After purification of two uricases, we investigated these uricase characterizations. There was no different Km value against oxygen between them. However, one uricase (UR9) showed lower Km value against uric acid than another uricase (UR2).Gain of function and loss of function experiments suggested that 6 base (GTAATG) motif in the 5' non-coding region of soybean uricase gene was a cis element for nodule uninfected cell specific expression.We produced the transgenic lotus plants for high level expression of PEPC or PPDK to analyze C or N metabolism in nodule.