Organic synthesis and Production based on Bioresources and Biocatalysis

近年来，在有机化学中出现了各种控制反应物种和反应条件的方法和理论。酶是个例外。化学家们将酶视为分子转化中的优秀试剂，并在合成有机化学中利用酶和微生物进行了许多努力。不用说，一个显著的优势是酶是催化剂，来自微生物、动物和植物的供应永远不会停止，因为资源可以以自我生产的方式繁殖。在2000-2001年的资助下，本研究从新的来源、新的生物量和新的发酵产物中获得了原料。其成果是：1)用抗Prelog酵母(Yamadazyma Farinosa)催化不对称还原合成了对映体对称富集物；2)利用酵母(Torulaspora DelBrueckii)催化的不对称还原和动力学拆分；3)澄清了脂肪酶催化的水解和酯化反应中的一些现象，以建立高效的动力学拆分；4)在糖偶联物的合成中的应用：N-乙酰氨基葡萄糖-CBZ-天冬氨酸；5)化学-酶法一体化工艺：(R)-2-萘甲氧基乙酸的合成。

A variety of methods and theory controlling the reaction species and conditions in organic chemistry has recently been developed. Enzymes are exception. Chemists look upon enzymes as elegant reagents in molecular transformation, and many efforts have been devoted on the utilization of enzymes and microorganisms in synthetic organic chemistry. Needless to say, a remarkable advantage is that enzymes are the catalysts whose supply from microorganisms, animals, and plants never quit, as the resources can be reproduced in a self-production manner. In this study supported by the grant-in-aid (2000-2001), moreover, the starting materials were secured from the new source, bio-mass and fermentation products.The achievements were; 1) Synthesis of enantioemrically enriched compounds by anti-Prelog yeast (Yamadazyma farinosa)-catalyzed asymmetric reduction; 2) Asymmetrization and kinetic resolution by means of a yeast (Torulaspora delbrueckii) catalyzed reduction; 3) Clarification of some phenomena observed in lipase-catalyzed hydrolysis and esterification toward the establishment of efficient kinetic resolution; 4) application on the synthesis of glycoconjugates: N-acetylglucosaminyl-Cbz-asparagine; 5) Integrated combined process of chemo-enzymatic procedure: synthesis of (R)-2-naphthylmethoxyacetic acid.