Ultra-highly effective utilization of lipid resources-Enzymatic syntheses of structured lipids-

1.作为该项目的前提，我们成功地分离和测定对称结构的三酰甘油，阿坝（其中A=正辛酸，B=多不饱和脂肪酸，PUFA），和不对称的，AAB（和/或BAA）的组合使用的银离子HPLC和手性HPLC。我们还成功地分离和测定了手性甘油二酯，AA（OH）和（OH）AA。采用两步法可以非常有效地合成对称结构的三酰甘油（阿坝），该方法包括用固定化南极假丝酵母脂肪酶对鲣鱼油进行乙醇分解，然后用固定化Rizomucor miehei脂肪酶对所得单酰甘油（OH）（PUFA）（OH）进行酯化。3.以含有13%二十二碳六烯酸和过量L-丝氨酸的蛋黄卵磷脂为原料，利用磷脂酶D的转磷脂酰化作用，成功地合成了磷脂酰丝氨酸。

1. As a prerequisite to this project, we succeeded in separation and determination of symmetrical structured triacylglycerol, ABA (where A=n-octanoic acid, B=a polyunsaturated fatty acid, PUFA), and asymmetrical ones, AAB (and/or BAA) by use of a combination of silver ion HPLC and chiral HPLC. We also succeeded in the separation and determination of chiral diacylglycerols, AA (OH) and (OH) AA.2. Symmetrical structured triacylglycerol, ABA, was able to be synthesized very effectively by a two-step method which was composed of ethanolysis of bonito oil with immobilized Candida antarctica lipase, and then esterification of the resulting monoacylglycerol, (OH) (PUFA) (OH) with immobilized Rizomucor miehei lipase.3. From egg yolk lecithin containing 13% docosahexaenoic acid and excessive L-serine, phosphatidylserine was able to be synthesized successfully by the transphosphatidylation action of phospholipase D.