Synaptische Transmission zwischen Mitralzelle und Körperzelle im olfaktorischen Bulbus der Ratte: Zwei-Photonen-Mikroskopie der synaptischen Calciumströme und simultane Elektrophysiologie in vitro

该项目旨在更好地了解早期嗅觉加工，其中二尖瓣细胞和颗粒细胞之间的大型树突状相互突触是主要参与者。我们的方法将基于同时应用双光子成像的钙动力学和传统的电生理学的突触传递在体外。考虑到在系统水平上理解灯泡中嗅觉信息处理的性质的长期目标，提出了以下研究路线：在研究了刺激二尖瓣细胞-颗粒细胞突触后的钙动力学之后，我们将测量情绪和觉醒相关的神经调节剂去甲肾上腺素对该突触中钙瞬变和突触传递的影响，因为有很强的神经调节投射到嗅球。最后，我们计划在一个更完整的电路的背景下，调查在二尖瓣细胞-颗粒细胞相互突触的传输，以进一步阐明离心输入信号处理在嗅球的影响。为此，我们将开发一种“一体化”切片制剂，其中还包含球外元素，即嗅觉皮层的一部分。

The proposed project aims at a better understanding of early olfactory processing, in which the large dendrodendritic reciprocal synapse between mitral cells and granule cells is a main player. Our approach will be based on simultaneous application of two-photon imaging of calcium dynamics and conventional electrophysiology of synaptic transmission in vitro. Given a long-term goal of understanding the nature of processing of olfactory information in the bulb at a systemic level, the following lines of research are proposed: Having investigated the calcium dynamics following stimulation of the mitral cell-granule cell synapse, we will measure the influence of the emotion- and arousal-related neuromodulator norepinephrine on calcium transients and synaptic transmission in this synapse, since there are strong neuromodulatory projections into the olfactory bulb. Finally, we plan to investigate transmission at the mitral cell-granule cell reciprocal synapse in the context of a more intact circuit, in order to further elucidate the influence of centrifugal inputs on signal processing in the olfactory bulb. To do so, we will develop an "integrative" slice preparation containing also extrabulbar elements, i.e. part of the olfactory cortex.