Existence of PGP9.5 in synoviocytes in horse joint and its application to clinical diagnosis

用抗蛋白基因产物(PGP)9.5的抗血清成功地对关节、滑囊和腱鞘中的B型滑膜细胞进行了选择性染色。此外，将整体制剂应用于这些组织使完全观察它们的分布和形状成为可能。滑膜囊的内表面覆盖有PGP9.5免疫反应的多核滑膜细胞和树突状突起。肌腱鞘内免疫反应细胞密集分布于肠系膜周围，其他部位免疫反应细胞较少，免疫反应细胞突起在表层形成较厚的一层。建立了检测正常关节和异常关节滑液中前列腺素P9.5含量的酶联免疫吸附试验(ELISA)。正常关节为0.329±0.033，关节内骨折为0.871±0.462。夹层骨软骨病(0.701±0.436)和炎性关节病(0.567±0.132)与对照组相比差异无统计学意义，但有升高趋势。因此，该方法对关节内骨折的诊断是有用的，并提示对于其他类型的关节疾病的诊断，可能需要更灵敏的两种抗体联合检测系统。

We succeeded in selectively staining type B synoviocytes in the joint, bursa synovialis and tendon sheath by use of an antiserum against protein gene product (PGP) 9.5. Furthermore, an application of whole mount preparations to these tissues made entire observation of their distribution and shape possible. Inner surface of the bursa synovialis was covered by PGP9.5-immunoreactive, multinuclear synoviocytes with dendritic processes. Immunoreactive cells in the tendon sheath were densely distributed around mesenterium, but fewer in other regions, where accumulated processes of immunoreactive cells formed a thick layer on the surface layer.The existence of PGP9.5 in synovial fluid was confirmed by immunoblot analysis for PGP9.5. We established an enzyme-linked immunosorbent assay (ELISA) to measure contents of PGP9.5 in the synovial fluid from normal and abnormal joints. PGP9.5 level in normal joints was 0.329±0.033, while it was evaluated as 0.871±0.462 in intra-articular fracture. Although the values for osteochondrosis dissecans (0.701±0.436) and inflammatory arthropathy (0.567±0.132) were not significantly different from the control level, they tended to be increased. Therefore, this assay is useful for diagnosis of intra-articular fracture and suggested that more sensitive assay system using two types of antibodies may be needed for diagnosis of other kinds of joint disorders.