Liver hematopoiesis and hematopoietic microenvironment - three dimensional reconstruction and immunohistochemical observation of CD markers

胎儿肝脏造血分为四个阶段：第1阶段，10天开始造血；第2阶段，1天和12天造血室体积扩大；第3阶段，13天和14天造血室体积达到峰值；第4阶段，15天后造血功能恢复。在I-II期，造血干细胞似乎从窦腔转移到原始肝细胞索中，在肝母细胞中形成集落。在第三阶段，造血集落形成椭球形的病灶作为造血的结构单位，然后红细胞病灶以脐带的形式出现。在IV期，这些索状病灶被破坏，在蜿蜒的肝窦上的肝细胞索内出现圆形孤立灶。第四期的造血病灶包括大量的粒细胞、淋巴细胞和红细胞。妊娠13天前肝母细胞体积基本不变，13天后逐渐增大。HI期的肝母细胞通过短的细胞质突起相互接触。&钙粘蛋白介导的粘附连接。在H-III期，除了粘附连接外，小桥粒出现将肝母细胞结合在一起，并且肝母细胞之间可以识别胆管的切面。IE期，胆管周围出现紧密连接和间隙连接，粘附连接、桥粒、紧密连接和间隙连接四种特化连接充分组织。为了区分淋巴细胞和髓系造血细胞并确定淋巴细胞亚类，使用了抗小鼠抗cd单克隆抗体。在对照染色中，抗体能够特异性地染色成人脾脏中的T淋巴细胞或B淋巴细胞，但在胎儿和新生儿肝脏中，无法获得特异性结果。用抗小鼠单克隆抗体进行免疫组化染色被认为是小鼠胎儿器官的未来任务。少

In fetal liver hematopoiesis, four stages were identified : Stage I, onset of hematopoiesis at 10 days, Stage II, expansion of the volume of the hematopoietic compartment at ll and 12 days, Stage III, peak in the volume of the hematopoietic compartment at 13 and 14 days, and Stage IV, involution of hematopoiesis after 15 days. During Stages I-II, hematopoietic stem cells appeared to move from the sinusoidal lumina into primitive hepatic cell cords to give rise to colonies among hepatoblasts. At Stage III, the hematopoietic colonies formed ellipsoidal foci as a structural unit of hematopoiesis, and then the erythropoietic foci appeared as cords. At Stage IV, these cord-shaped foci became disrupted, and round solitary foci appeared within the hepatic cell cords on meandering sinusoids. Hematopoietic foci in Stage IV contains numerous granulocytes and lymphocytes as well as erythroblasts.The hepatoblast volumes appeared constant until 13 days of gestation, and then showed a gradual increa … More se. Hepatoblasts at stage HI made contact with one another by short cytoplasmic projections. ＆cadherin mediated adherens junctions were found. At stage H-III, in addition to the adherens junctions, small desmosomes appeared to bind hepatoblasts together, and section profiles of biliary canaliculi could be recognized between hepatoblasts. At the IE stage, both tight junctions and gap junctions appeared around the biliary canaliculi, and four types of specialized junctions, i. e., adherens junctions, desmosomes, tight junctions and gap junctions, appeared fully organized.In order to differentiate lymphocytes from myeloid hematopoietic cells and to determine lymphocytesubclasses, anti-mouse anti-CD monoclonal antibodies were used. In control staining, the antibodies were ableto stain T or B lymphocytes specifically in adult spleens, but, in fetal and neonatal livers, specific results could no be obtained. Immunohistochemical stainings with the anti-mouse monoclonal antibodies were considered to be future tasks in mouse fetal organs. Less