Analysis of integrins in lymphnode metastasis of bladder cancer

我们在动物模型中比较了整合素在膀胱癌及其淋巴转移中的表达，以阐明整合素在膀胱癌淋巴转移中的作用。采用人膀胱癌UM-UC-3和UM-UC-13细胞系，采用足垫埋植法建立淋巴转移动物模型，用抗整合素亚基的单抗进行免疫组织化学染色(组织ABC法和组织荧光法)。我们发现整合素在原发足垫病变和淋巴结转移中的表达没有差异。此外，UM-UC-3和UM-UC-13未产生高转移细胞。我们还试图用UM-UC-3和UM-UC-13从膀胱种植中建立高转移细胞，但没有发现膀胱种植的淋巴转移。综上所述，其他肿瘤细胞株应用于相同的实验模型，或今后应研究临床种类。

We compared integrins expression of bladder cancers to those of their lymphnode metastasis in animal model, in order to clarify the role of integrins in the lymphnode metastasis of bladder cancer. The animal model of lymphnode metastsis was created by means of foot pad implantation method using human bladder cell lines, UM-UC-3 and UM-UC-13, and each integrin subunit was detected immuno-tissue stain (tissue ABC method and tissue fluorescent method) using monoclonal antibody against each integrin subunit. We found no difference in integrin expression between primary foot pad lesion and lymphnode metastasis. In addition, highly metastatic cells was not created from UM-UC-3 and UM-UC-13. We also tried to create highly metastatic cells from bladder implantation using UM-UC-3 and UM-UC-13, however, no lymphnode metastasis was found from bladder implantation. In conclusion, other tumor cell lines should be used in the same experimental model, or clinical speciemens should be investigated, in the future.