Mechanism of ischemia-reperfusion injury

本研究的目的是探讨巨噬细胞是否与缺血再灌注损伤有关。在大鼠皮瓣缺血再灌注模型上，用免疫组织化学方法研究巨噬细胞的作用。将股动脉和股静脉夹在腹壁血管上方和下方11h后松开。再灌流后12h组织学检查显示巨噬细胞侵入皮瓣，再灌流后24h可见白细胞侵入。再灌流后48h，皮瓣内过氧化物酶活性达到高峰。在右腹上腹皮瓣抬高之前，静脉注射二氯亚甲基二磷酸(DMDP)。然而，DMDP对缺血再灌注损伤的作用尚未被认识到。

The purpose of this study is to evaluate whether macropharge is related to ischemia-reperfusion injury. The concern of macropharge was investigated by immuno-histochemical study in the ischemia-reperfusion model of rat skin flaps. The femoral artery and vein were clamped above and below the epigastric vessels for 11 h and then the clamp was released. Histological examination 12 h after reperfusion showed macropharge invasion into the flap and leukocyte invasion was recognized 24 h after reperfusion. Peroxidase activity in the flap presented a peak 48 h after reperfusion.Dichloromethylene diphosphonate (DMDP) was administered intravenously just before elevation of the right abdominal epigastric flap. However the effect by DMDP to ischemia-reperfusion injury was not recognized.