The ultrastructure of osteoclast ruffled border and resorption lacuna

我们通过速冻、深蚀刻和旋转阴影复制品在超微结构水平上研究了破骨细胞的褶边。在冷冻替代切片中，据Akisaka等人1997年报道，皱褶边缘呈指状或叶状。然而，褶边的三维结构尚不清楚。深蚀刻电子显微镜显示了与冷冻替代切片相同的图像，但也提供了三维信息。在深蚀刻的复制品中，可以分辨出复杂的褶边。当断裂面穿过皱褶边缘时，可以看到手指和叶状的网状细胞骨架。网状网络作为细胞质核心充满细胞内。这些细丝似乎与皱边膜的内表面有接触。褶皱边缘的膜内颗粒与网状网络、接触之间的直接关系可能与骨吸收的极化和激活与质膜的关联是不可分割的。另一方面，基底侧膜未出现质膜的夹带。

We studied the ruffled border of osteoclasts at ultrastructural levels by quick-frozen, deep-etched, and rotary shadowed replicas. In freeze substituted section, the ruffled border was reported finger-shaped or foliate by Akisaka et al., 1997. However, three-dimensional structure of the ruffled border are not known. The deep-etched electron microscopy revealed the same image as freeze-substituted sections, but also provided three-dimensional information. Complicated infoldings of ruffled border was distinguished in the deep-etched replica.When the fracture plane passed through the ruffled border, the reticular network as cytocskeletal of the fingers and foliate became visible. The reticular network fills the innercellular as a cytoplasmic core. These filaments appeared to do contact with the inner surface of the ruffled border membrane. The direct relation between the intramembranos particles of the ruffled border and the reticular network, contact may be integral to association of polarization and activation with the plasma membranes for bone resorption. On the other hand, baso-lateral membran does not appeared infoldings of plasma membrane.