GROWTH OF METASTATIC CACNER CELLS IN THE LYMPH NODES AND ITS REGULATION BY LYMPHOCYTES

为了控制转移淋巴结，我们在体内和离体检查，得到以下结果：IL-2激活的淋巴细胞对癌细胞的细胞毒活性通过抗癌药物（^<137>Cs）预处理癌细胞而增强，淋巴细胞与抗癌药物联合使用具有较强的细胞毒活性。癌细胞的凋亡诱导与癌细胞的Mn-SOD活性呈负相关，il -2活化淋巴细胞的凋亡部分依赖于癌细胞中的活性氧。Mn-SOD反义转染诱导细胞高水平凋亡，促凋亡蛋白增加，抗凋亡蛋白减少。结内注射PLM和CDDP后，所有患者均出现PR或NO。关于淋巴结淋巴细胞（LNL）；LNL亚群与外周血淋巴细胞（PBL）不同，由高B细胞群（~ 60%）和低T细胞群（~ 10%）组成。LNL表达的表面抗原与PBL相似。LNL的细胞毒活性明显低于PBL。IL-2或INF-γ不能成功上调LNL的细胞毒活性，但在体外，IL-2+IFN-γ/OK-432+外周血单核细胞（树突状细胞）可以成功上调LNL的细胞毒活性。

To control the metastatic lymph nodes, we examined in and ex vivo and obtained following results.1. The cytotoxic activity of IL-2 activated lymphocytes against cancer cells was increased by pretreatment of cancer cells with anticancer drugs or ^<137>Cs, and a combination of the lymphocytes and anticancer agents brought a strong cytotoxicity.2. Apoptosis induction of cancer cells was inversely associated with Mn-SOD activity of the cancer cells and their apoptosis by IL-2-activated lymphocytes partially depended on reactive oxygen species in the cancer cells. Mn-SOD antisense transfection induced high level apoptosis with an increases of proapoptotic proteins and a decrease of antiapoptotic proteins.3. The intranodal injection of PLM and CDDP brought PR or NO in all patients.4. Concerning lymph node lymphocytes (LNL) ; LNL subpopulations differed from those in peripheral blood lymphocytes (PBL), consisting of a high population of B cells (〜60 %) and low population of T cells (〜10 %). LNL expressed surface antigens similar with those in PBL. The cytotoxic activity of LNL was largely lower than that of PBL. The upregulation of the lowered cytotoxic activity of LNL was not succeeded by IL-2 or INF-γ but in vitro succeeded by the addition of IL-2+IFN-γ/OK-432+peripheral blood monocytes (dendritic cells).