Study of neuropathic pain for rats

神经病理性疼痛的机制是复杂的。我们通过行为学测试和西洛他唑的治疗，研究了三种动物模型在Spraogue-Dawley大鼠上的差异。糖尿病组注射链脲佐菌素，对照组注射0.1M柠檬酸缓冲液。慢性压迫性损伤(CCI)大鼠的手术方法与Bennetts的模型相同。对照组接受双侧假手术，作为非CCI-CFA大鼠。完全弗氏佐剂(CFA)大鼠按照埃利亚夫的模型进行手术。将糖尿病(DM)大鼠、非DM大鼠、CCI大鼠、非CCI-CFA大鼠、CFA大鼠分为两组。其中一组给予含有西洛他唑的微丸，另一组仅给予微丸。每周对所有大鼠进行行为测试。这些测试研究了机械性痛觉过敏、冷痛觉过敏和机械性痛觉过敏。所有模型均出现机械性痛觉超敏反应。西洛他唑仅对DM组的机械性痛觉过敏有效。仅CCI组出现冷痛和机械性痛觉过敏，西洛他唑对此无明显影响。我们定义了每种模型的机制、作用持续时间和神经。重要的是，我们要单独处理每个病例，了解神经病理性疼痛等机制不能简单地治疗

The mechanisms of neuropathic pain are complicated. We investigated differences in three animal models on Sprague-Dawley rats using a behavior test and treatment of cilostazol. The diabetic group received an injection of streptozotocin, and the control rats received an injection of 0.1 M citrate buffer. Operations were done on Chronic Constriction Injury (CCI) rats in the same way as with Bennetts' model. The control rats, which received bilateral sham procedures, were used as non-CCI-CFA rats. Operations were done on Complete Freund's Adjuvant (CFA) rats as prescribed by Eliav's model. Each group of diabetes mellitus (DM) rats, non-DM rats, CCI rats, non-CCI-CFA rats, and CFA rats were subdivided into two groups. One group was given pellets containing cilostazol and the other group was given only pellets. All rats were given behavior tests each week. The tests investigated mechano-allodynia, cold allodynia and mechano-hyperalgesia. Mechano-allodynia was induced in all models. Cilostazol was effective on mechano-allodynia only in the DM group. Cold allodynia and mechano-hyperalgesia was induced only in the CCI group, which showed no change with cilostazol. We defined the mechanisms, duration of effect, and nerves for each models. It is important that we deal with each case individually and that understand mechanisms such as neuropathic pain cannot be treated simply