Study on environmental pollution with mutagens in high mortality areas by lung cancer

采用铂铑催化柱和荧光检测器对30份表层土壤样品中的1,3-二硝基芘（DNP）、1,6-DNP和1,8-DNP进行了定量分析。在所有检测的土壤样品中均检测到DNP异构体，其在土壤样品中的浓度从几pg/g不等。在某土壤样品中检测到3-硝基苯并蒽酮（NBA）为主要诱变剂，具有较强的诱变性。为了明确3-NBA在环境中的分布，我们建立了大气颗粒物和表层土壤中3-NBA的分析方法。3-NBA用高效液相色谱法部分纯化，荧光检测器高效液相色谱法定量。近畿地区环境空气中3-NBA浓度为几pg/m3，近畿、东海和关东地区土壤样品中3-NBA浓度在几ng至几百ng/g之间。

1,3-Dinitropyrene (DNP), 1,6-DNP and 1,8-DNP in 30 surface soil samples were quantified by high performance liquid chromatography equipped with a platinum/rhodium catalyst column and a fluorescence detector. DNP isomers were detected in all soil samples examined, and their concentration in soil samples ranged from a few pg/g of soil. 3-Nitrobenzanthrone (NBA) was detected as a major mutagen in a soil sample that showed strong mutagenicity. To clarify the distribution of 3-NBA in the environment, we developed an analytical method of 3-NBA in airborne particles and surface soil. 3-NBA was partially purify by HPLC and quantified by HPLC with a fluorescence detector. Concentrations of 3-NBA in ambient air in the Kinki area were a few pg/m3, and those in soil samples from the Kinki, Tokai and Kanto areas ranged from a few ng to a few hundred ng/g of soil.