Analysis of functional group distribution of intramembranous globular proteins in plasma membrane of lymphocyte adhesion sites on hydrophilic/ hydrophobic ABA-type microphase-separeted structure surfaces with different lamellar spacings by transmission el

本研究的目的是评价phema - st - phema aba型嵌段共聚物（HSB）表面的最佳片层间距，并利用具有良好自我/非自我选择功能的淋巴细胞进行生物相容性表现。在8nm、16nm和43nm处制备了HSB的片层间距。以PSt和PHEMA-PSt无规共聚物（HSR）表面为对照。用微球柱法测定淋巴细胞与聚合物表面的相互作用。室温固定孵育时间后，透射电镜（TEM）分析HSB表面淋巴细胞黏附部位质膜膜内球状蛋白的官能团。然后，利用图像处理分析仪定量测定了其功能基团的直径，特别是膜内球蛋白外膜小叶的氨基（NH_2）和内膜小叶的巯基（SH）的直径。在16nm片层间距的HSB表面，淋巴细胞黏附部位的膜内球状蛋白中NH_2和SH的直径与完整淋巴细胞的直径相同。另一方面，在HSB表面8nm和43nm片层间距处，淋巴细胞黏附部位的膜内球状蛋白中NH_2和SH的直径与完整淋巴细胞的不同。8nm和43nm片层间HSB表面的NH_2和SH均呈部分重叠。有趣的是，虽然8nm片层间的SH直径与43nm片层间的SH直径相同，但8nm片层间的NH_2直径高于43nm片层间的NH2直径。粘附在PSt和HSR表面的淋巴细胞的功能基团，由于淋巴细胞的坏死反应，无法通过透射电镜分析。结果表明，HSB表面的片层间距大小对其生物相容性的表现起着重要的作用。结果表明，16nm片层间距的HSB表面抑制了淋巴细胞膜的流动性。少

The aim of this research was to evaluate the optimum lamellar spacing of PHEMA-PSt-PHEMA ABA-type block copolymer(HSB) surface for bio-compatibility manifestation using lymphocyte with good self/non-self selectional function. The lamellar spacings of the HSB were prepared at 8nm, 16nm and 43nm. PSt and PHEMA-PSt random copolymer(HSR) surfaces were used as controls. Interaction between lymphocytes and polymer surfaces was carried out using Microsphere Column Method. After a fixed incubation time at room temperature, the functional groups of intramembranous globular proteins in the plasma membrane of the lymphocyte adhesion sites on the HSB surfaces were analyzed by Transmission Electron Microscopy(TEM). Then, diameters of the functional groups were quantitatively evaluated, using an image-processor analyzer: especially, the diameters of amino groups(NH_2) of the outer membrane leaflet and the diameters of sulfhydryl groups(SH) of the inner membrane leaflet, in the intaramenbranous globu … More lar proteins. The diameters of the NH_2 and SH in the intramembranous globular proteins of the lymphocyte adhesion sites, on the HSB surfaces of the 16nm-lamellar spacings were the same value as those of the intact lymphocytes. On the other hand, the diameters of the NH_2 and SH in the intramembranous globular proteins of the lymphocyte adhesion sites, on the HSB surfaces of the 8nm and 43nm-lamellar spacings weren't the same value as those of the intact lymphocytes. The NH_2 and SH on the HSB surfaces of the 8nm and 43nm-lamellar spacings were observed to partly cap. Interestingly, while the diameters of the SH on the 8nm-lamellar spacings were the same value as that of the SH on the 43nm-lamellar spacings, the diameters of the NH_2 on the 8nm-lamellar spacings indicated a higher value more than the diameters of the NH2 on the 43nm-lamellar spacings. The functional groups of the lymphocytes adhered to the PSt and HSR surfaces, couldn't be analyzed by TEM due to a necrotic reaction of the lymphocytes. It was indicated that the lamellar spacing size of the HSB surfaces played an important role for the bio-compatibility manifestation. And then, it was suggested that the HSB surfaces with the 16nm-lamellar spacing inhibited the fluidity of the lymphocyte plasma membrane. Less