DEVELOPMENT OF BIOCHEMICAL IC CHIPS

研究了用于未来微纳化学器件的生化IC芯片。与传统的研究项目相比，我们采取了系统的研究方法进行开发。1)开发了新的制造工艺来创建真正的三维微纳结构。最初在Ikuta实验室开发的微/纳米立体光刻工艺在这个项目中得到了进一步的发展。采用激光诱捕技术制备了纳米针和纳米镊子。实验验证了平滑远程驱动和ft牛顿尺度力传感。2)成功开发了新型生化IC芯片芯片组，并对其功能进行了验证。利用先进的微泵芯片成功合成了无细胞蛋白。在没有任何活细胞的情况下，已经用DNA合成了几种蛋白质。这一功能不仅是后基因组研究的关键技术，也是订制医学的基础。3)均质机芯片在不破坏细胞蛋白的情况下，在微观尺度上破坏细胞和组织。提出了一种简便有效的生化集成电路芯片间耦合方法。硅橡胶联轴器密封紧密。这些结果直接有助于真正的全微分析过程和设备。4)利用微立体光刻技术研制出人工肾装置。微型化和大鼠试验在长仓教授的实验室成功进行。5) Sokabe教授和Naruse教授提出并验证了利用微流体的细胞生物学新技术。由于通过这种新方法获得了许多生命科学的新知识，因此明确了诸如生化IC芯片之类的微化学器件可以为基础生命科学和生物医学应用做出贡献。

Biochemical IC chips for future micro/nano chemical device have been investigated. We took a systematic research approach for development compared with conventional research project.1) New fabrication process to create real three dimensional micro/nano structures has been developed. The micro/nano stereo lithography process originally developed in Ikuta's laboratory was further advanced for this project. Nano scale needle and tweezers were fabricated and driven by laser trapping. Smooth remote drive and ft Newton scale force sensing were demonstrated experimentally.2) New chip-sets of biochemical IC chips have been successfully developed and their functions were verified. Cell-free protein synthesis with advanced micro pump chips was succeeded. Several proteins have been synthesized from DNA without any living cell. This function is the key technology for not only post genome research but also order-made medicine based.3) Homogenizer chips to destroy cell and tissue in micro scale without damaging cell protein. Simple and effective coupling method between biochemical IC chips was developed. The silicon-rubber coupling can make a tight sealing. These results directly contribute to real total micro analysis process and devices.4) Artificial kidney device utilizing micro stereo lithography has been developed. Miniaturization and rat test were carried out successfully in Prof. Nagakura's lab.5) New technique for cell biology by using micro fluidics has been proposed and verified by Prof. Sokabe and Prof. Naruse. Since many new knowledge in life science were obtained through the new approach, it was made clear that micro chemical devices such as biochemical IC chips can contribute to basic life science as well as biomedical applications.