权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of the nano device for medical use based on the Biochemical IC

基于生化IC的医疗用纳米器件的开发

基本信息

批准号：
15206027
负责人：
IKUTA Koji
金额：
$ 32.2万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

We studied the novel conceptual device, "Biochemical IC chip," from the basic material stage to the applicable cell-fusion chip stage under systematic approach. The purposes of this research are diversification and application to biology, which all the distances of the various synthesis and analysis in the biochemistry area could downsize to micro-size domain.Biochemical IC chip for cell-five protein synthesis, originally developed and verified as use for luciferase, a luminous protein of firefly in the year of 2001, was evolved to be synthesized general and well known marker protein Green fluorescence protein (GFP) continuously for along-term period. Through this new evidence, utility and generality of the Biochemical IC chip-set for cull-free protein synthesis was verified. As a basic technology of a mien) device with a built-in cell, we found the condition which the cell could be cultivated in the micro device among the appointed time, by studying several point, hunt up the photocur … More able resin with biocompatibility, surface modification, surface structure, fabrication strategy of the microstructure, and so on.This basic study is essential because photo-initiator, containing in the photocurable polymers, prevent cell growth on the surface of the device. We achieved the processing resolution of less than 10 micrometer, which high-resolution is required to construct the free-surface microstereolithography, essential to fabricate hybrid structure for biochemical IC chip. This new technology is contributed to not only acquire 10-micrometer-diameter micro pipe, but also improvement of the cell adhesion.We proposed and developed "optically driven nano-machine," which is controlled by the laser trapping remotely The nano-machine has movable elements and is fabricated by high-speed two-photon-absorption microstereolithography to realize a cell operation under the microscope in advance. We also verified its availability by fabricating nano-tweezer, nano-needle of 2 D.O.F.,10 micrometer-length nano-manipulator of 3 D.O.F, and so on.We also developed three-dimensional microfabrication system for biodegradable polymers, especially poly (lactic acid), for the purpose of the implantable use as a drug release chip of the biochemical IC. The resolution attained are 50 micrometer, but the fabricated device has good biocompatibility, because we never employ toxic solvents. Less

我们在系统的方法下，从基本材料阶段到应用细胞融合芯片阶段，对新型概念器件--“生化IC芯片”进行了研究。本研究的目的是多样化和应用于生物学，在生物化学领域的各种合成和分析的所有距离都可以缩小到微米领域。2001年最初开发并验证用于萤火虫发光蛋白荧光素酶的五细胞蛋白质合成生化IC芯片进化为长期连续合成通用的、著名的标记蛋白绿色荧光蛋白(GFP)。通过这一新的证据，验证了用于无CULL蛋白质合成的生化IC芯片组的实用性和通用性。作为内置细胞培养装置的一项基本技术，我们找到了在指定时间内在微型装置中培养细胞的条件，通过对几个点的研究，寻找到了光敏的…树脂具有更强的生物相容性、表面改性、表面结构、微观结构的制备策略等。由于光引发剂中所含的光固化聚合物阻止了细胞在器件表面的生长，所以这一基础研究是必不可少的。我们实现了小于10微米的加工分辨率，这是构建生物化学IC芯片混合结构所必需的自由表面微立体光刻所需的高分辨率。这项新技术不仅有助于获得直径为10微米的微管，还有助于改善细胞的粘附性。我们提出并开发了一种由激光捕获远程控制的光驱动纳米机器。纳米机器具有可移动的元件，通过高速双光子吸收显微立体光刻技术制造，实现了提前在显微镜下进行细胞操作。通过制作纳米镊子、2自由度纳米针、10微米长3自由度纳米机械手等验证了其有效性，并开发了可生物降解聚合物，特别是聚乳酸的三维微加工系统，用于植入型生物化学集成电路的药物释放芯片。该器件的分辨率可达50微米，但由于不使用有毒溶剂，因此具有良好的生物相容性。较少