Development of the nano device for medical use based on the Biochemical IC

本研究以系统的方法，从基本材料阶段到应用的细胞融合芯片阶段，对新概念装置“生化IC芯片”进行研究。本研究的目的是生物学的多样化和应用，将生物化学领域的各种合成和分析的距离缩小到微米级的范围。用于细胞五蛋白合成的生物化学IC芯片，最初于2001年开发并验证用于萤火虫发光蛋白荧光素酶，在长期的进化过程中，不断地合成出通用的、众所周知的标记蛋白绿色荧光蛋白（GFP）。通过这一新的证据，实用性和通用性的生物化学IC芯片组的无淘汰蛋白质合成进行了验证。作为一种内置细胞的微型装置的基本技术，我们通过对几个方面的研究，找到了细胞在微型装置中能够在规定时间内培养的条件，探索了光固化的方法， ...更多信息 生物相容性、表面改性、表面结构、微结构的制备策略等方面的研究，这些基础研究是必要的，因为光固化聚合物中含有的光引发剂可以阻止细胞在器件表面的生长。我们实现了小于10微米的加工分辨率，这是构建自由表面微立体光刻所需的高分辨率，是制造生化IC芯片混合结构所必需的。我们提出并研制了一种由激光捕获远程控制的“光驱动纳米机器”，该机器具有可移动的元件，通过高速双光子吸收微立体光刻技术制作，实现了在显微镜下对细胞的操作。我们还通过制作纳米镊子、二维纳米针，我们还开发了可生物降解聚合物，特别是聚乳酸的三维微加工系统，用于生物化学集成电路的药物释放芯片的植入。所获得的分辨率为50微米，但所制造的器件具有良好的生物相容性，因为我们没有使用有毒溶剂。少

We studied the novel conceptual device, "Biochemical IC chip," from the basic material stage to the applicable cell-fusion chip stage under systematic approach. The purposes of this research are diversification and application to biology, which all the distances of the various synthesis and analysis in the biochemistry area could downsize to micro-size domain.Biochemical IC chip for cell-five protein synthesis, originally developed and verified as use for luciferase, a luminous protein of firefly in the year of 2001, was evolved to be synthesized general and well known marker protein Green fluorescence protein (GFP) continuously for along-term period. Through this new evidence, utility and generality of the Biochemical IC chip-set for cull-free protein synthesis was verified. As a basic technology of a mien) device with a built-in cell, we found the condition which the cell could be cultivated in the micro device among the appointed time, by studying several point, hunt up the photocur … More able resin with biocompatibility, surface modification, surface structure, fabrication strategy of the microstructure, and so on.This basic study is essential because photo-initiator, containing in the photocurable polymers, prevent cell growth on the surface of the device. We achieved the processing resolution of less than 10 micrometer, which high-resolution is required to construct the free-surface microstereolithography, essential to fabricate hybrid structure for biochemical IC chip. This new technology is contributed to not only acquire 10-micrometer-diameter micro pipe, but also improvement of the cell adhesion.We proposed and developed "optically driven nano-machine," which is controlled by the laser trapping remotely The nano-machine has movable elements and is fabricated by high-speed two-photon-absorption microstereolithography to realize a cell operation under the microscope in advance. We also verified its availability by fabricating nano-tweezer, nano-needle of 2 D.O.F.,10 micrometer-length nano-manipulator of 3 D.O.F, and so on.We also developed three-dimensional microfabrication system for biodegradable polymers, especially poly (lactic acid), for the purpose of the implantable use as a drug release chip of the biochemical IC. The resolution attained are 50 micrometer, but the fabricated device has good biocompatibility, because we never employ toxic solvents. Less