Preparation of the oligo-DNA modified electrode and application of recognition for DNA segment

制备了一种基于多孔碳电极的新型固定化DNA电极，用于研究DNA与化合物之间的相互作用。通过电化学方法对该电极的特性进行了评价，并研究了DNA与锇配合物的相互作用。在该电极上（电极表面；0.66 - 2.37 cm^2）， DNA固定在0.26 × 10^<-4>-1.23 × 10^<-4> mol cm^<-2>。观察到DNA杂交的相对标准偏差为11% （n=4）。发现三（联吡啶基）锇（II/III）离子（锇配合物）与主要或次要凹槽相互作用。利用这种锇络合物，一个DNA序列可以被超过12个相同的序列所识别。由于固定化的变化较大，仅采用物理吸附的固定化方法在分析中存在一定的局限性。正在进行的固定处理的改进将使这些电极能够应用于各种相互作用的详细研究。

A novel immobilized DNA electrode based on a porous carbon electrode was prepared to study interactions between DNA and chemical compounds. The characteristics of this electrode were evaluated by electrochemical methods, and then the interaction between DNA and an osmium complex was studied. On this electrode (electrode surface ; 0.66 - 2.37 cm^2), DNA was immobilized at 0.26 x 10^<-4>-1.23 x 10^<-4> mol cm^<-2>. Hybridization of DNA was observed with a relative standard deviation of 11% (n=4). The tris(bipyridyl)osmium(II/III) ion (osmium complex) was found to interact to major or minor grooves. Using this osmium complex, a DNA sequence could be recognized with more than 12 mer of the same sequence.It was found that the immobilization using only physical adsorption gave some limitation in the analysis because of the relatively large variation of immobilization. Improvement of immobilization treatment, which is in progress, would enable these electrodes to be applied for detailed study of various interactions.