Garlic as new anti-cancer and chemopreventive functional food

大蒜(Allium Sativum L.)据报道，它的成分可以降低患癌症的风险。在这个项目中，我们将大蒜定性为一种新的化学预防和抗癌食品。二烯丙基三硫(DATS)及其类似物对人结肠癌细胞株HCT-15增殖的影响DATS可显著抑制Hct-15细胞的增殖(IC50=5.0μM)，而二烯丙基单硫化物(如二烯丙基单硫化物；DAS)和二硫化合物(如二烯丙基二硫化物；DADS)对细胞的增殖无明显影响。根据ALK(En)基碳链长度的不同，ALK(En)基三硫化物处理的细胞表现出抑制细胞增殖的趋势。DATS抑制细胞周期于G2/M期，同时伴有Cyclin B1蛋白水平升高，并诱导载脂蛋白…通过激活caspase-3，细胞内出现更多的上睑下垂。DATS抑制间期细胞聚合。体外微管蛋白浊度测定。结论：1.DATS抑制微管聚合，但不抑制微管解聚。烯丙基硫化物的化学预防作用；烯丙基硫化物对I、II相药物代谢酶的影响。给Wistar品系大鼠灌胃14天。在I相酶活性方面，DAS(100μ/kg)可显著提高细胞色素P450(GYP)2E1活性，而DADS和DATS对其、肝细胞色素P450水平和细胞色素P1A1/2活性无明显影响。在大鼠急性肝损伤模型中，DATS(10μμ/kg)和DADS(100μμ/kg)均能显著降低CCl_4诱导的II相酶活性，DATS(10μm ol/kg)和DADS(100μm o l/kg)均能显著减轻CCl_4诱导的II相酶活性的损伤。因此，DATS可能是大蒜油中保护机体免受日常生活中自由基分子损伤的重要因素之一。较少

Garlic (Allium sativum L.) and its components have been reported to reduce the risk of cancer. In this project we have characterized garlic as new chemopreventive and anti-cancer food.1. Anti-cancer effect of allyl sulfides ; structure-function relationship and their mechanisms involved in anti neoplastic activityIn this study we investigated the effects of diallyl trisulfide (DATS) and its analogue alk(en)yl sulfides on proliferation of HCT-15, human colon cancer cell line. DATS significantly inhibited the proliferation of HCT-15 cells (IC_<50>=5.0μM), while both alk(en)yl monosulfides (e.g. diallyl monosulfide ; DAS) and disullfides (e.g. diallyl disulfide ; DADS) did not show any effect on the cell proliferation. Depending on the alk(en)yl carbon chain length, alk(en)yl trisulfide treated cells showed the tendency to inhibit the cell proliferation. DATS inhibited the cell cycle progression at G2/M phase which accompanied elevation of cyclin B1 protein level, and also induced the apo … More ptosis in the cells via activation of caspase-3. DATS inhibited the polymerization of the cells in interphase. In vitro tubulin turbidity assay. DATS inhibited the microtubule polymerization, but not microtubule depolymerization.2. Chemopreventive effect of allyl sulfide ; The effect of allyl sulfides on the phase I and II drug-metabolizing enzymes.Allyl sulfides were administered i.p. as a bolus to Wistar strain rats for 14 days. As to the phase I enzymes, DAS (100μmol/kg) significantly increased cytochrome P450 (GYP) 2E1 activity, whereas DADS and DATS did not affect it or the hepatic total CYP level or CYP1A1/2 activity. With respect to the phase II enzymes, DATS (10μmol/kg) and DADS at a 10-fold higher dose (100μmol/kg) significantly increased the activities of glutathione S-transferase, quinone reductase, and glutathione peroxidase ; whereas DAS did not.In the carbon tetrachloride (CCl_4)-induced acute liver injury model of rats, either DATS (10μmol/kg) or DADS (100μmol/kg) significantly reduced the injury caused by the induction of phase II enzymes with CCl_4. In conclusion, DATS may be one of the important factors in garlic oil that protects our body against the injury caused by radical molecules encountered in daily life. Less