To Establish Cytometric Epidemiology by Measuring Benzo[a]pyrene-DNA Adducts as an Example

我们的结果如下：(1)标准化的肺癌死亡率与日本西部空气中的NO2含量和温度显著相关(例如，每10万人中有1.9名男性死亡和0.42名女性死亡，同时增加1 ppb的NO2和1℃的温度)。(2)开始吸烟时过滤器内的温度受到室温的影响，过滤器内的温差随着时间的推移而减小。(3)将不同浓度的BPDE暴露在培养细胞中，在37℃诱导DNA修复，在40℃通过细胞死亡。(4)福冈，熊本、冈山和札幌之所以被选中，是因为气温和肺癌死亡率的差异。用抗BPDE-DNA加合物的5D11抗体，用流式细胞仪检测了439名正常男性志愿者外周血淋巴细胞中BPDE-DNA加合物的水平。由于外周淋巴细胞包括BPDE-DNA加合物阳性细胞和阴性细胞，我们计算了加合物阳性细胞的比例、平均加合物水平及其产物作为总加合物水平。肺癌死亡率高、气温低的札幌市阳性细胞比例明显高于其他城市(p&lt；0.001)，死亡率高、气温高的福冈，其加合物阳性细胞组较高和较低。札幌市的BPDE-DNA加合物总水平也高于其他城市(p&lt；0.001)。然而，我们没有发现BPDE-DNA加合物在年轻人和老年人之间，吸烟者和不吸烟者之间的显著差异，以及平均加合物水平的地区差异。

Our results were as follows :(1) Standardized lung cancer mortality was significantly correlated with NO_2 content in the air and temperature in Western Japan (e.g., a simultaneous increase of 1 ppb NO_2 and 1℃ of temperature corresponding to 1.9 male death and 0.42 female death per 100,000 population).(2) Temperature in the filter was affected by room temperature at the beginning of smoking, and a difference in temperature in the filter reduced with time.(3) An exposure of graded concentrations of BPDE to cultured cells induced a DNA repair at 37℃ And cell death through apoptosis at 40℃.(4) Fukuoka, Kumamoto, Okayama, and Sapporo were chosen because of a difference in temperature and lung cancer death rate. The BPDE-DNA adduct levels in peripheral lymphocytes from 439 normal male volunteers were measured using 5D11 antibody specific to BPDE-DNA adduct by flow cytometry. As peripheral lymphocytes included BPDE-DNA adduct-positive cells and negative-cells, we calculated proportions of adduct-positive cells, mean adduct levels, and their products as a total adduct level. The proportion of positive cells was significantly higher in Sapporo, high lung cancer death rate and low temperature, than other cities (p<0.001), and Fukuoka, high death rate and high temperature, contained both higher and lower adduct-positive cell groups. Total BPDE-DNA adduct levels were also higher in Sapporo than the other cities (p<0.001). However, we failed to find significant differences in BPDE-DNA adduct between the younger and elder or between smokers and nonsmokers and regional differences in mean adduct level.