Study on the regulation of sucrase-isomaltase mRNA in the small intestine

在非肥胖型2型糖尿病模型Goto-Kakizaki（GK）大鼠中检查了长期（6个月）给予含伏格列波糖的饮食混合物（10 ppm）对肠道二磷酸脱氢酶活性的影响。伏格列波糖治疗组GK大鼠的餐后血糖水平显著低于未治疗组GK大鼠（190±19 vs. 250±25 mg/dl，P < 0.01; 1 h，212±23 vs. 256±20，P < 0.05; 2 h），并且麦芽糖酶、蔗糖酶和异麦芽糖酶的活性在整个6个月的伏格列波糖治疗期间保持显著较低。伏格列波糖处理组蔗糖酶-异麦芽糖酶（SI）复合物的蛋白和mRNA表达均显著增加。治疗6个月后停止伏格列波糖给药。SI复合物的mRNA水平和蛋白质水平在给药中断期间变得正常化，并且在治疗停止后1个月，disdispylidase活性几乎增加到未治疗组的水平。然而，在重新给药1天后，双磷酸腺苷酶活性再次受到显著抑制。这些结果表明，伏格列波糖可以改善葡萄糖耐量，因为它抑制了二硫键苷酶的活性，尽管增加了它们在肠道上的表达，此外，伏格列波糖可以通过中断和重新给药而可逆和重现。2002爱思唯尔科学爱尔兰有限公司保留所有权利。

The effect of long-term (6 months) administration of voglibose in a dietary mixture (10 ppm) on intestinal disaccharidase activity was examined in non obese type 2 diabetes model Goto-Kakizaki (GK) rats. The postprandial blood glucose level in voglibose-treated GK rats was significantly lower than in untreated GK rats (190±19 vs. 250±25 mg/dl, P < 0.01; 1 h, 212±23 vs. 256±20, P < 0.05; 2 h), and the activities of maltase, sucrase, and isomaltase remained significantly lower throughout the 6 months of voglibose treatment. The expressions of protein and mRNA of sucrase-isomaltase (SI) complex were significantly higher in voglibose-treated GK rats. Voglibose administration then was stopped after 6 months of treatment. The mRNA level and protein level of the SI complex became normalized during the interruption of drug administration, and disaccharidase activities increased almost to the level of the untreated group 1 month after treatment was stopped. After 1 day of re-administration of the drug, however, disaccharidase activities again became significantly inhibited. These results indicate that voglibose may improve glucose tolerance since it inhibits activities of disaccharidases in spite of increasing the expression of them on intestine, furthermore voglibose may be reversible and reproducible through interruption and re-administration.* 2002 Elsevier Science Ireland Ltd. All rights reserved.