Visualization of brain perfusion by contrast-enhanced ultrasonography : a preliminary study in rabbit

我们评价了超声显像增强介质（半乳糖帕米氨酸：Levovist）对家兔脑灌注显像的有效性。我们在兔子的颅骨上做了一个洞，我们用它来做声窗。采用脑右中动脉闭塞法制备缺血模型。采用HDI5000 （ATL）配线性5-12Mhz探头，获得静脉注射造影剂（150mg/体）前后的功率多普勒图像和谐波图像。使用造影剂，功率多普勒图像显示小动脉清晰，但未观察到脑实质增强。在谐波图像上观察到脑灌注增强。第一次造影剂的增强效果最强。冲洗非常快，没有观察到对比介质的池化。在缺血模型中，通过造影剂可以清楚地观察到缺血侧脑灌注不足。结论：超声造影可观察脑灌注情况，超声造影可用于评价脑缺血性疾病。

We evaluated the usefulness of contrast-enhance media of ultrasonography (galacutose parmitine acid : Levovist) to visualize the brain perfusion in rabbit.We made a hole in skull bone of the rabbit under anesthesia, and we used it for acoustic window. Ischemic model was made by occlusing the right middle cerebral artery. Power Doppler Images and Harmonic images before and after intravenous injection of contrast media (150mg/body) were obtained using HDI5000 (ATL) equipped with linear type 5-12Mhz probe. Using contrast media, small arteries were clearly visualized by Power Doppler images, but enhancement of brain parenchyma was not observed. Enhancement of brain perfusion was observed on Harmonic images. Enhancement was strongest on the first pass of the contrastmedia. Washout was very fast and pooling of contrast media was not observed. In ischemic models, hypoperfusion of the brain on the ischemic side was clearly observed by using contrast media. We concluded brain perfusion can be observed on Harmonic ultorasonographic imaging by using contrast media, and contrast enhanced ultrasonography may useful for evaluating ischemic diseases in human brain.