Mitochondria injuries of Peritoneal Mesothelial Cells induced by oxidative stress

活性氧（ROS）引起的氧化应激被认为参与多种疾病的发病机制。用8- OHdG对氧化应激对腹膜的病理影响进行了初步研究。8- OHdG免疫组化染色在健康对照组间皮细胞中呈弱染色，而在硬化性腹膜炎患者间皮细胞和血管壁中呈强染色。证实腹膜炎浸润细胞中存在8- OHdG。CAPD溶液中8- OHdG水平与透析时间成比例升高，与年龄无关，在腹膜炎患者中较高。因此，CAPD溶液中的8- OHdG水平可能作为腹膜炎症和间皮细胞损伤的临床标志。随后，我们研究了高葡萄糖浓度（HG组，214mM）对大鼠培养间皮细胞的影响。用二氯荧光素对ROS的产生进行了可视化观察和检测。暴露于HG后，间皮细胞比对照组产生更多的ROS。这些结果表明，线粒体极易受到氧化应激的影响，因为它们是细胞内ROSNext的主要来源，我们从形态学上研究了急性腹膜炎期间的新血管生成。采用激光扫描共聚焦显微镜，用荧光单纯格里菲亚1凝集素观察腹腔毛细血管的形态。平行的亲本毛细血管之间的分支吻合形成不规则的花边状网状结构，而不是单独的直线连接。腹膜炎模型大鼠腹膜表面可见细胞浸润，部分细胞表达CD34。免疫组化显示Flk-1、Flt-1和Tie-2在这些细胞中表达。这些结果可能代表了一个新的和有前途的策略，治疗腹膜功能障碍的新血管形成的临床应用

Oxidative stress by reactive oxygen species (ROS) is thought to be involved in the pathogenesis of various diseases. The pathological effect of oxidative stress on the peritoneum was studied basically with 8- OHdG. Immunohistochemical staining of 8- OHdG was weakly detected in the mesothelial cells of the healthy control, whereas strong staining was observed in the mesothelial cells and vascular walls of patients with sclerosing peritonitis. The presence of 8- OHdG in infiltrative cells in peritonitis was confirmed. The 8- OHdG levels in CAPD solution increased in proportion to the duration of dialysis irrespective of age, and they were high in the patients with peritonitis. Thereforem the 8- OHdG level in CAPD solutions might possibly be used as a clinical marker of inflammttion of the peritoneum and impairment of mesothelial cellsSubsequently, the effect of a high glucose concentration (HG group, 214mM) on rat culture mesothelial cells was investigated. The ROS production was visuali … More zed and checked by Dichlorofluorescein diacetat. Upon exposure to HG, the mesothelial cells produced more ROS than those of the control group. These results suggest that the mitochondria were extremely vulnerable to oxidative stress because they were a major intracellular source of ROSNext, neoangiogenesis during acute peritonitis was investigated morphologically. Fluorescent Griffonia simplicifolia 1 lectin was used to visualize the pattern of capillaries in the peritoneum by laser scanning confocal microscopy. Branching anastomoses between the parallel parent capillaries formed an irregular lace- like meshwork rather than individual straight interconnections. In the peritonitis model rats, cell infiltration was recognized on the surface of the peritoneum, and some of these cells expressed CD34. Immunohistocemistry revealed expression of Flk-1, Flt-1, and Tie-2 in these cells. These results may represent a new and promising strategy for dealing with peritoneal dysfunction by clinical application to new vessel formation Less