Possible Role of Serotonin in Hepatic Ischemia and Reperfusion Injury in Rat

背景已知Srotoninn可诱导血小板聚集，是一种强大的血管收缩药。结论1.血栓形成引起的血管狭窄和血管收缩加重了周围组织和器官的缺血。目的探讨选择性5-羟色胺受体拮抗剂(5HTra)在大鼠肝脏缺血再灌注损伤中的作用，探讨5-羟色胺对肝缺血再灌流(HIR)后肝脏微循环障碍的影响，以及选择性5-羟色胺受体拮抗剂(5HTra)在此条件下的作用。材料与方法选用8周龄雌性S/D大鼠。夹闭肝内侧叶蒂30、60、90min，造成部分肝缺血。5HTra组在缺血前15min和再灌流前15min分别以10 mg/kg的剂量腹腔注射5HTra。测定…的范围检测血清天冬氨酸转氨酶(U/L)、血清丙氨酸转氨酶(U/L)、血清乳酸脱氢酶(U/L)水平。分别于缺血前、缺血前30、60、90、120分钟从心脏抽血。比较5HTra组和对照组缺血再灌流后肝酶的变化，并用5-羟色胺特异性抗体研究5-羟色胺在缺血再灌注肝脏中的表达，然后进行5-羟色胺的免疫组织化学染色。结果腹腔注射选择性5-羟色胺能受体拮抗剂可显著抑制肝脏再灌流后酶的释放，缺血90min再灌流60min后，肝窦内检测到5-羟色胺，提示5-羟色胺在缺血再灌注损伤的发病机制中起重要作用。结论5-羟色胺可能在肝脏缺血再灌注损伤的发病机制中起重要作用。较少

1. backgroundSrotoninn is known to induce platelet aggregation and to be a strong vasoconstrictor. Vascular stenosis by the formed thrombi and the vasocontraction aggravates ischemia of peripheral tissues and organs.2. aimThe aim of this study was to evaluate possible role of serotonin in hepatic ischemic reperfusion injury in rat by the selective S2-serotonergic receptor antagonist (5HTra).This study was conducted to investigate the infuence of serotonin on the hepatic microcirculation disturbance after hepatic ischemia and reperfusion (HIR) injury and to evaluate the effect of the selective S2-serotonergic receptor antagonist (5HTra) under these conditions.3. materials and methods8 week old Female S/D rats were used. Partial liver ischemia was performed by clamping the pedicles of the medial lobe for 30, 60, 90 minutes. In the 5HTra group, 5HTra was administered at a dose of 10mg/kg by intraabdominal injection 15min before ischemia and 15min before reperfusion.To determine the extent … More of hepatocellular damage during reperfusion, the levels of serum aspartate transaminase (U/L), serum alanine transaminase (U/L) and serum lactate dehydrogenase (U/L) were measured. Blood was withdrawn from the heart before ischemia, 30, 60, 90, 120 minutes. Change in the liver enzymes after ischemia reperfuson in the 5HTra group were compared with those in the control group.In addition, using specific antibody to the serotonin, to study 5-HT expression in the liver subjected to ischemia and reperfusion, immunochemical staining for 5-HT was then performed.4. resultThe intraabdominal administration of selective S2-serotonergic receptor antagonist a significant inhibition of the postreperfusion release of the liver enzymes.Serotonin was detected in sinusoid in the liver subjected to 90 min of ischemia followed by 60min of reperfusion.Thus ,we conclude that serotonin is strongly involved in the pathogenesis of ischemia reperfusion injury.5. conclusionThese and other results suggested that serotonin might play critical roles in the pathogenesis of hepatic ischemia and reperfusion injury. Less