Development of highiy bioactive artificial bone by bioactive material anci growth factor

本研究的目的是通过在生物活性陶瓷中添加生长因子1：TAK-778TAK778增强骨结合能力是一种新形成的低分子成骨物质，它可能对成骨细胞的分化起到调节作用。将该材料应用于生物活性陶瓷与骨的界面，结果表明TAK-778提高了陶瓷的骨结合强度。2.将多孔AW或多孔HA(孔隙率为70%，平均孔径为200 nm)的多孔AWCylinder植入兔股骨的骨髓腔内，并按时间顺序进行分析。AW被连续吸收，12个月时残留物质相当于起始材料的30%左右，而HA几乎没有被吸收。AW组材料孔内新生骨体积大于HA组。AW-骨结合在骨重建和材料吸收过程中保持不变。因此，多孔AW具有比HA更快的吸收速度和更大的成骨能力，更适合作为生长因子的载体。3.AW-b-FGF复合体的成骨能力：将多孔AW圆柱体在高真空下浸泡在b-FGF液中，植入兔股骨内。尽管观察到成纤维细胞生长因子组比对照组(未加b-成纤维细胞生长因子)有更多的血管形成，但骨形成的数量是相似的

The purpose of this study is to create an artificial bone that has a comparable bone-forming ability to natural bone by adding growth factors to bioactive ceramics1: Enhancement of bone-bonding ability by TAK-778TAK778 is a newly formed low molecular agent, which may exert regulatory effects on osteoblast differentiation. This agent was applied to the interface between bioactive ceramics and bone at implantation, which demonstrated that TAK-778 enhanced the bone bonding strength of the ceramics2: Absorption of porous AWCylinders of porous AW or porous HA (porosity 70% and mean pore diameter 200 urn for both materials) were implanted into the bone marrow cavity of rabbit femurs, and analyzed chronologically. AW was absorbed continuously, and at 12 months the residual material corresponded to about 30% of the starting material, whereas HA was hardly absorbed. The volume of newly formed bone in the material pores was higher with AW than with HA. AW-bone bonding was maintained during bone remodeling and material absorption. Thus porous AW showed faster absorption and greater bone ingrowth than HA, suggesting better suitability for a carrier of growth factors3: Boneforming ability of AW-b-FGF complexPorous AW cylinders were soaked in the solution of b-FGF under high vacuum and were implanted into rabbit femur. Although more vascular formation into the pores was observed in FGF group than control (AW without b-FGF), the amounts of bone formation were comparable