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Gene expression ground biomaterials implanted into bone

植入骨骼的基因表达研磨生物材料

基本信息

批准号：
11680840
负责人：
NEO Masashi
金额：
$ 2.3万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

Tissue response around β-tricalciumphosphate (TCP, absorbable material) or poltmethylmethacrylate (PMMA, non-bioactive material) particles implanted into rat tibiae was analyzed by in stu hybridization with type I collagen (COL), osteonectin (ON), osteocalcin (OC) and osteopontin (OPN) RNA probes. Specimens were collected at 3, 5, 7, 10 days after operation. Holes without implantation were used as control. In all groups, new bone was formed centripetally and all four kinds of mRNA were expressed in activated osteoblasts with a similar pattern. A COL signal was expressed most strongly and widely, and was detected at the peripheral region of the hole at day 3. The other three mRNAs were also expressed in bone forming osteoblasts by day 7. These active osteoblasts were often observed on the surface of the TCP but not on the PMMA particles. The activities of osteoblasts in the control and the TCP groups were similar, whereas that in the PMMA group was weaker. On the other hand, OPN-positive and COL-negative cells were observed on the surface of the PMMA particles strongly for a long time. They were speculated to be macrophages and osteoclasts because they showed acid phosphatase activity. These cells were rarely observed on the TCP particles and were not observed in the control. These findings suggest that the early cell responses and gene expressions reflect the bioactivity or biocompatibility of the implanted biomaterials.

用Ⅰ型胶原（COL）、骨连接素（ON）、骨钙素（OC）和骨桥蛋白（OPN）RNA探针原位杂交，分析β-磷酸三钙（TCP）或聚甲基丙烯酸甲酯（PMMA）植入大鼠胫骨后的组织反应。分别于术后3、5、7、10 d取材。未植入的孔用作对照。在所有组中，新骨向中心形成，所有四种mRNA在活化的成骨细胞中以相似的模式表达。COL信号表达最强和最广泛，并在第3天在孔的周边区域检测到。到第7天，其他三种mRNA也在骨形成成骨细胞中表达。这些活跃的成骨细胞通常在TCP的表面上观察到，但在PMMA颗粒上没有观察到。对照组和TCP组成骨细胞活性相似，而PMMA组较弱。另一方面，在PMMA颗粒的表面上长时间强烈地观察到OPN阳性和COL阴性细胞。它们被推测为巨噬细胞和破骨细胞，因为它们显示酸性磷酸酶活性。在TCP颗粒上很少观察到这些细胞，并且在对照中未观察到。这些结果表明，早期细胞反应和基因表达反映了植入生物材料的生物活性或生物相容性。