Human follicular development flollowing xenotransplantation of human pnarian cortex into immune deficient mice

我们研究了类固醇生成酶的免疫组织化学表达和定位的非病理人类卵巢移植后异种移植到非肥胖糖尿病严重联合免疫缺陷（NOD-SCID）小鼠。通过对类固醇生成酶的免疫组织化学抗体研究了异种移植到NOD-SCID小鼠中的人卵泡。在这些移植物的腔前卵泡中，类固醇生成酶、p450 SCC、p450 c17和3β-HSD仅存在于卵泡膜细胞层中，观察结果与非病理性人类卵巢皮质的结果一致。细胞色素p450芳香化酶和雌激素受体在这些移植物中未检测到，这些卵泡不具有优势卵泡的特征。这些结果提示，NOD-SCID小鼠异种移植后，人卵泡中类固醇生成酶的表达。类似于非病理性人类卵巢。豪韦 ...更多信息 r，这些卵泡不具有优势卵泡的特征。已知其发育为黄体。此外，我们成功地将人卵巢皮质异种移植到NOD/SCID/g cnull小鼠后，成功地产生了成熟的人graafian卵泡。移植后10周，我们通过每天腹腔注射人绝经期促性腺激素刺激卵巢组织14天。移植后12周从受体小鼠中取出卵巢移植物。8只移植小鼠中有2只。从卵巢囊内的移植组织获得直径为16 -18 mm的人Graafian卵泡。细胞色素p450芳香化酶和雌激素受体在小鼠卵巢囊中发育的颗粒层和人卵泡膜层中均有高水平的表达。这些结果表明，这些卵泡具有相似的特征，作为一个正常的绝经前周期人类卵巢的优势卵泡。少

We studied the immunohistochemical expression and localization of steroidogenic enzymes in the graft of non-pathological human ovary following xenogenic transplantation into a non-obese diabetic severe combined immune deficient (NOD-SCID) mice. Human follicles following xenotransplantation into NOD-SCID mice were studied by immunohistochemistry antibodies against steroidgenic enzymes. In the pre antral follicles of these grafts, steroidogenic enzymes, p450 scc,p450 c17 and 3β-HSD were present in only the the theca cell layer, observations which are consistent with the findings of non-pathological human ovarian cortex. Cytochrome p450 aromatase and estrogen receptor, however, were not detected in these grafts, and these follicles did not possess specific feature of a dominant follicle. These findings suggest that the expression of steroidogenic enzymes in humnan follicles following xenogenic transplantation into NOD-SCID mice. is similar to that of non-pathological human ovaries. Howeve … More r, these follicles do not possess features of dominant follicles. which are known to develop into the corpus luteum. Furthermore, we succeed the successful generation of mature human graafian follicles following xenotransplantation of a human ovarian cortex into a NOD/SCID/g cnull mouse. Ten weeks after transplantation, we stimulated the ovary tissue through daily intraperitoneal injections of human menopausal gonadotropin for 14 days. Ovarian grafts were removed from recipient mice twelve weeks after transplantation. In 2 out of 8 transplanted mice. human graafian follicles l6-18 mm in diameter were obtained from transplanted tissues within the ovarian bursa. High levels of cytochrome p450 aromatase and estroqen receptor expression were observed in both the granulosa and the human graafian follicle theca layer developed in tne murine ovarian bursa. These results suggest that these follicles have similar characteristics as the dominant follicles of a normal premenopausal cycling human ovary. Less