Functional recovery after nerve transplantation to optic nerve

将周围神经移植到视神经断端，可重建视路。有报道称，恢复后的视觉通路可以传导明暗等视觉信息。在这项研究中，我们试图评估恢复后的视觉系统可以处理的运动信息，使用带有运动刺激的预脉冲抑制作为预脉冲。为了获得这样的结果，动物的视野必须是固定的。因此，我们试图在α-氯醛糖麻醉下记录脉冲前抑制。然而，正常对照组大鼠在这种情况下不能获得明显的预脉冲效应。接下来，我们尝试使用穿梭箱回避任务来评估对运动刺激的检测。正弦波栅的运动被用作条件刺激。无条件的刺激是从盒子的地板上传来的震动。在动作速度为40、20、1.5、0.5度/S的情况下，获得了清晰的学习曲线，第5天的避避率为55-70%。对9只移植的大鼠进行了以白色LED为预脉冲的预脉冲抑制试验。1只大鼠表现出抑制作用的趋势。这只大鼠是在穿梭箱中用20度/S的运动刺激进行测试的。回避率随着训练的进行而增加。经过20度/S的测试后，同样进行了40度/S的训练，但没有观察到学习效果。另一只移植大鼠对LED预脉冲没有显示预脉冲效应，作为穿梭箱任务的对照。避税情况并未见有改善。这些结果表明，一些移植的大鼠可能能够感觉到运动

Reconstruction of visual pathway can be achieved by the peripheral nerve transplantation to the cut end of the optic nerve. It has been reported that the restored visual pathway can conduct some visual information such as light-dark. In this research we have tried to evaluate motion information can be processed by the restored visual system, using prepulse inhibition with a motion stimulus as a prepulse. To obtain this result, animal's visual field should be fixed. Thus we have tried to record prepulse inhibition under alpha-chloralose anesthesia. However, clear prepulse effects could not be obtained in this condition in normal control rats. Next, we have tried to evaluate detection of motion stimulus using shuttle-box avoidance task. A motion of sine-wave gratings was used as a conditioned stimulus. Unconditioned stimulus was a shock from the floor of the box. Clear learning curves have been obtained with a motion speed of 40, 20, 1.5,0.5 deg/s, showing avoidance rate of 55-70% at the 5th day of training. Nine transplanted rats have been tested using prepulse inhibition with a white LED as a prepulse. One rat showed a tendency of inhibition effect. This rat was tested in the shuttle-box using a motion stimulus of 20 deg/s. Avoidance rate increased as progress of training sessions. After the test with 20 deg/s, same training has been done with a motion speed of 40 deg/s. However, no learning effect was observed. Another transplanted rat, showing no prepulse effect to LED prepulse was tested as a control for shuttle-box task. No improvement of avoidance rate has been observed. These results suggest that some transplanted rat might be able to detect motion