The studies of the tooth germ differentiation and the mineralization of chondrocytes in vitro

1)Meckel‘s软骨诱导分化的研究(1)Meckel’s软骨形成下颌关节韧带：为阐明Meckel‘s软骨在小鼠体内形成该韧带的机制，本实验从细胞增殖、分化及基质钙化等方面观察了表皮生长因子对软骨细胞的影响。(2)热休克诱导的Meckel‘s软骨细胞死亡：我们检测了热休克处理诱导的Meckel’s软骨细胞的死亡是凋亡还是坏死，并特别注意热休克蛋白(HSP)-70和-27的表达，以分析热休克蛋白(HSP)-70和-27在细胞凋亡中的作用。(3)…对Meckel‘s软骨细胞向脂肪细胞的表型转化更多的鸡血清：当鸡血清(CKS)存在于培养液中时，可调节体外培养的Meckel‘s软骨细胞向脂肪细胞的表型变化。2)软骨细胞向成骨细胞的表型转化：为了分析成骨细胞的表型转化，在细胞和器官培养体系中培养了小鼠和兔的下颌骨髁状突(MMC)，并进行了形态学和生物化学相结合的检测。结果表明，培养条件下MCC细胞的形态和生化修饰发生在细胞形态水平和标记基因表达水平。3)器官培养系统中牙根的形成：牙根的形成与Hertwig上皮根鞘(Hertwig‘s上皮根鞘)的延长密切相关。在器官培养体系中，观察外源性IGF-I、抗IGF-I抗体对小鼠磨牙早期伸长的影响。较少

1)The studies on the differentiation of Meckel' s cartilage(1) Formation of the shenomandibular ligament by Meckel's cartilage : To clarify the mechanism of formation of this ligament by Meckel's cartilage in mouse, we examined the effects of epidermal growth factor (EGF) on the chondrocytes in terms of the proliferation and differentiation of cells and calcification of the matrix in vivo and in vitro. EGF enhanced DNA synthesis and the proliferation of Meckel's chondrocytes, inhibited the differentiation of cells to chondrocytes and then induced phenotypic changes in fibroblastic cells.(2) Cell death in Meckel's chondrocytes by heat-shock treatment : We examined whether the cell death induced in Meckel's chondrocytes by heat-shock treatment is apoptosis or necrosis, and particular attention was paid to the expression of heat shock protein (HSP) -70 and -27 to analyze the involvement of HSPs in apoptosis.(3)Phenotypic conversion to adipocyes from Meckel's chondrocyte by the treatment o … More f chick serum : When present in the culture medium, chick serum (CKS) modulated the phenotypic change from chondrocytes of Meckel's cartilage to adipocytes in vitro. The adipocytes are induce simply and high frequency by agents in chick serum.2)The phenotypic conversion of chondrocytes to osteoblastic cells : In order to analyze the phenotypic conversion of chondrocytes, mandibular condyles (MMC) of mice and rabbits were cultured under cell and organ culture systems, and then examined by a combination of morphological and biochemical procedures. It results suggest that, morphologically and biochemically, cellular modification in MCC cells under culture conditions occurs at a cellular morphological level and also at marker-gene-expression level.3)Dental root formation in organ culture system : Dental root formation is closely related the elongation of Hertwig's epithelial root sheath(HERS). The study examined the effects of exogenous IGF-I, anti-IGF-I antibody on the early HERS elongation of mouse molar in organ culture system. Less