Can periodontopathic bacteria induce the cytokines production that cause atherosclerosis in ApoE knockout mice ?

循环系统疾病如动脉粥样硬化涉及非常复杂的因素，如高脂血症、肥胖、吸烟和遗传因素。近年来已经阐明，微生物感染侵入血管内皮细胞，并在侵入部位触发动脉粥样硬化。因此，动脉粥样硬化被认为与微生物感染诱导的炎症性疾病有关。流行病学研究表明牙周病与缺血性动脉疾病之间存在关系。许多研究小组提出了牙周病相关生物膜和血管疾病之间的联系机制。我们在动脉粥样硬化病变的样本中检测到牙周病性齿垢密螺旋体。我们从包埋的动脉疾病样本中提取DNA，并研究了6个动脉瘤病变中齿垢锥虫16 S rRNA的存在。当样品用特异性抗齿垢锥虫抗体染色时，我们观察到聚集的抗原颗粒与动脉瘤中泡沫细胞内和周围的抗体反应。与心脏外科中心合作，我们从动脉硬化病变部位的冠状动脉壁斑块样本中提取DNA，并研究牙龈卟啉单胞菌、伴放线放线杆菌、坦那氏菌、直弯曲杆菌和齿垢坦那氏菌的特异性DNA的存在。结论冠心病患者牙周组织中牙龈卟啉单胞菌和直肌的检出与牙周组织中牙龈卟啉单胞菌和直肌感染密切相关。我们证明T.人脐静脉上皮细胞分泌IL-8和ICAM-1。这些结果表明，牙周病细菌影响细胞因子网络，发挥了重要作用，控制稳态的结果触发或涉及炎症反应。

Circulatory disorders such as atherosclerosis involve very complex factors such as hyperlipidemia, obesity, smoking, and genetic factors. It has been clarified in recent years that microbial infections invades the vascular endothelial cells, and triggers atherosclerosis at the invasion site. In consequence, atherosclerosis has been considered to relate a microbial infection-induced inflammatory disorder. Epidemiological studies have shown the relationship between periodontal disease and ischemic arterial disease. Postulated mechanisms to link periodontal disease-associated biofilm and vascular disease are shown in many research groups. We detected periodontopathic Treponema denticola in samples of atherosclerosis lesions. We extracted DNA from embedded arterial disease samples and investigated the presence of T.denticola 16S rRNA in 6 aneurysm lesions. When the samples were stained with specific anti-T.denticola antibody, we observed aggregated antigenic particles reacting with the antibody in and around foam cells in aneurysms. In cooperation with a heart surgery center, we extracted DNA from plaque samples from coronary arterial walls at the sites of arterosclerosis lesions, and investigated the presence of specific DNA of Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Tannerella forsythensis, Campylobacter rectus and T.denticola. We concluded that detections of P.gingivalis and Crectus from coronary disease sites were closely related with their infections in periodontal sites. We demonstrated that the cells of T. denticola produced IL-8, and ICAM-1 from human umbilical vein epithelial cells. These findings suggest that periodontopathic bacterial affect the cytokine network that plays a significant role to control homeostasis results in the trigger or relates to inflammatory responses.