Development of Sensing System of Human Chemical Senses Using EQCM Method

EQCM测量系统是在第一年建成的。然后，研究了电化学测量方法和QCM测量方法的基本特点。第二年，研究了在石英谐振器上形成脂分子感应层的方法。利用该传感元件进行了电化学测量和QCM测量。作为敏感层的脂膜形成在敏感元件的表面，即石英谐振器。利用该传感元件实现了电化学测量与QCM测量的同时进行。问题出在EQCM分析仪的软件结构和单元结构上。通过改变实验程序、数据处理和细胞结构的材料，解决了这一问题，探索了形成对味觉亚基…具有高感受性的脂膜的方法高稳定度的石英谐振器表面有更多的NCES。采用在水溶液中分散少量脂类，使石英谐振器表面物理吸附脂类分子的方法。通过这种物理吸附方法对苦味物质过去形成敏感层的方法，使QCM测量的灵敏度提高了一位数以上。然后，使用EQCM分析仪和石英谐振器将电化学测量和QCM测量同时结合起来，使脂质物理吸附。然而，EQCM分析仪的响应没有与QCM单次测量的结果相适应，而且EQCM分析仪没有获得足够的灵敏度，估计不能通过驱动电路的不同来获得足够的响应。然后，将电化学方法从循环伏安法改为脉冲伏安法。结果，获得了对强吸附苦味物质的选择性响应，这是本研究的目的之一。较少

The EQCM measurement system was constructed during year first. Afterwards, basic characteristics of the electrochemical measurement method and the QCM measurement method were examined. Next year, the method of being form the sensing layer of lipid molecule on a Quartz resonator was examined. An electrochemical measurement and the QCM measurement were done by using the sensing element. The lipid membrane as the sensing layer was formed on the surface of the sensing element that was a Quartz resonator. Combining the simultaneous of electrochemical measurement and the QCM measurement was done by using this sensing element.At first, stability was lacked when QCM measurement was executed. The problem turned out in the structure of software and the cell of EQCM analyzer. It straightened out that problem by changing the material of the experiment procedure, data processing and the cell structure.It searched for the method of forming the lipid membrane with high receptivity to the taste substa … More nces on the surface of the Quartz resonator with high stability. The method of distributing a small amount of lipid in aqueous solution, and making the surface of the Quartz resonator physically adsorb the lipid molecule was adopted. The sensitivity improvement of one digit or more on the QCM measurement was obtained from a past formation method of sensing layer for the bitter substance by this physical adsorption method. Then, the electrochemical measurement and the QCM measurement were combined simultaneously using the EQCM analyzer and the Quartz resonator to make the lipid physically adsorbed. However, the response of the EQCM analyzer did not adjust to the result of QCM single measurement, and enough sensitivity was not obtained from EQCM analyzer.It has been estimated not to obtain an enough response by the difference of the driving circuit. Then, the electrochemical method was changed from cyclic voltammetry to the pulse voltammetry. As a result, a selective response to a strong adsorbent bitter substance that was one of the purposes of this research was obtained. Less