Development and Application of High Performance Capillary Electrophoresis Using a New Capillary Fabricated by Laser

研制了一种新型的激光烧蚀毛细管，并将其应用于毛细管电泳柱后反应和阿达玛变换毛细管电泳柱后荧光检测。在毛细管的中心开了一个小孔。在多次进样和后标记反应中，小孔分别作为进样和标记剂的进气口。在多次进样中，孔被浸入样品溶液中，而通常用于进样的毛细管的末端被浸入缓冲溶液中。在进样口和毛细管末端之间持续施加高电位，允许将样品溶液引入分离毛细管中。通过在毛细管两端之间施加根据阿达玛编码调制的较高电位，将缓冲溶液注入分离毛细管。该方法成功地用于阿达玛变换毛细管电泳联用吸收检测器和激光诱导荧光检测器的多样品进样。在柱后反应中，在荧光检测器的上游打孔。将该孔浸入标记试剂溶液中。毛细管电泳法分离的分析物区带与从孔中引入的标记剂混合，然后标记剂与分析物反应，并对标记的分析物进行荧光检测。

A novel capillary fabricated by laser ablation was developed and applied to Hadamard transform capillary electrophoresis and post-column reaction for fluorescence detection in capillary electrophoresis. A small hole was fabricated at the center of the capillary. The small hole functions as an inlet port for the sample and labeling reagent in multiple sample injection and post-labeling reaction, respectively. In multiple sample injection, the hole is immersed in a sample solution and the end of the capillary that is usually employed for sample introduction is immersed in a buffer solution. A high potential is continuously applied between the injection port and the end of the capillary, which allows the sample solution to be introduced into the separation capillary. By application of a higher potential modulated according to a Hadamard code between both ends of the capillary, the buffer solution is injected into the separation capillary. This method was successfully employed for multiple sample injection in Hadamard transform capillary electrophoresis equipped with an absorbance detector and a laser-induced fluorescence detector. In post-column reaction, the hole was made at upstream of a fluorescence detector. The hole is immersed in a labeling reagent solution. Analyte zones separated by capillary electrophoresis were mixed with the labeling reagent introduced from the hole, followed by the reaction of the labeling reagent with analytes and fluorescence detection of the labeled analytes.