Development of the total analysis systems combining the microchip and capillary

（1）毛细管组装微芯片（CAs-CHIP）制备方法的研究将方形毛细管嵌入到PDMS平板的点阵微通道网络中，制备了CAs-CHIP。在整个过程中，为了避免溶液泄漏，最终键合是关键步骤。我们开发了使用旋涂PDMS预聚物的键合方法，并成功地实现了最终的键合。（2）流体控制-生物传感系统的开发流体控制是开发高功能化微流控器件的重要任务。本文采用温度响应聚合物固定化毛细管作为阀控元件，成功实现了微通道内流体的流动控制。为了将该体系应用于生物传感，我们将固定化酶毛细管与阀控毛细管结合使用，取得了阀控和传感操作的初步结果。（3）多离子传感系统的研制为了研制多离子传感系统，我们制备了多种离子传感器， ...更多信息 通过将含有不同离子载体的增塑PVC膜附着到毛细管的内表面来密封毛细管。从选择性和响应范围两个方面评价了每根毛细管的离子传感性能，发现每根毛细管都有足够的性能用于血清分析。将钠、钾、钙离子传感毛细管嵌入芯片，成功制备了多离子传感芯片。（4）前处理-毛细管电泳分离系统的研制本文采用CAs-CHIP作为毛细管电泳分离的前处理附件。设计了具有T型接头的微通道，在下游设计了阀门交叉结构，并连接了分离毛细管。通过使用含有高分子量和低分子量物质的样品溶液，对基于扩散的小分子分离、进样和CE分离进行了测试，然后成功地实现了CE分离前的蛋白质去除型预处理。少

(1)Development of the fabrication method of capillary-assembled microchip (CAs-CHIP)CAs-CHIP was fabricated by embedding the square capillaries into lattice microchannel network of PDMS plate. Among the whole procedure, final bonding is a crucial step in order to avoid solution leakage. We have developed the bonding method using spin-coated PDMS prepolymer, and successfully achieved the final bonding.(2)Development of the flow control-biosensing systemFlow control is an important task for developing highly-functionalized microfluidic device. Here we used thermo-responsive polymer-immobilized capillary as valving parts, and fluid flow control inside microchannel was successfully achieved. In order to apply this system for biosensing, we used enzyme-immobilized capillary together with valving capillary, and preliminary results of valving and sensing operation was achieved.(3)Development of the multi-ion sensing systemIn order to develop multi-ion sensing system, we prepared various ion-s … More ensing capillaries by attaching the plasticized PVC membranes containing different ionophores to the inner surface of the capillaries. Ion-sensing performance of each capillary was evaluated in terms of selectivity and response range, and found that each capillary has enough performance for the application to serum analysis. Sodium, potassium and calcium ion-sensing capillaries were embedded onto the chip, and successfully prepared the multi-ion sensing chip.(4)Development of the pretreatment-capillary-electrophoresis separation systemHere CAs-CHIP was used as the pretreatment attachment of the capillary-electrophoresis separation. The microchannel having T-junction was designed, and at the down stream, valving cross structure was designed and separation capillary was connected. By using the sample solution containing high and low molecular weight species, diffusion-based separation of small molecules, injection and CE-separation was tested, then, protein removal-type pretreatment before CE separation was successfully achieved. Less