Integration and visualization of genome information in Lepidoptera

我们于2003年利用雌性特异性STSs筛选的W-BAC建立了家蚕性染色体鉴定方法，并在鳞翅目中建立了首个BAC-FISH系统。前一种方法已于2005年用于求解多性染色体系统。改良的BAC-FISH程序使用基因定位的家蚕BACs作为探针，使我们能够鉴定每28个二价。此外，每条染色体的两到四个绿色或红色荧光标记探针的组合使我们能够通过其标记模式明确识别28个二价染色体的核型，这在80多年来是不可能的。通过研究，我们完成了包含512个BAC contigs的第二代分子连锁图谱，与网络上发表的大多数独特基因相对应。这为突变体对应基因的图谱克隆提供了可能。除了少数例外，BAC-FISH信号的位置与遗传分析检测到的定位位点一致。由于差异可能是由于三点分析的错误造成的，我们最好重新计算结果。

We have developed Bombyx mori sex chromosome identification method and first BAC-FISH system in Lepidoptera by means of W-BAC selected by female specific STSs in 2003. The former method has been applied to resolve multiple sex chromosome systems in 2005. Modified BAC-FISH procedure using genetically mapped B.mori BACs as probes allow us to identify each 28 bivalents. Furthermore, the combination of two to four either green or red fluorescence-labeled probes per chromosome enables us to recognize unequivocally each of the 28 bivalents of the B.mori karyotype by its labeling pattern, which have been impossible for more than 80 years. Through the research we have accomplished 2nd generation molecular linkage map including 512 BAC contigs, which correspond to most unique genes published on the web. These may feasibly facilitate the map-based cloning the genes corresponding to mutants.The BAC-FISH signal localities coincide with their mapping loci detected by genetic analysis with a few exceptions. Since the discrepancy may be caused by mistakes of three-point analysis, we had better re-calculate the results.