Sensing device using integrated nanospaces

我们开发了一种基于介孔薄膜（MTFs）的新型化学传感装置，其中嵌有氟离子团和掩蔽剂。使用该传感器，已成功地证明了水溶液中金属离子的高选择性检测。通过将前驱体溶液浇铸到微孔底部的硅微升板上沉积MTFs。将8-羟基喹啉-5-磺酸（QS）作为一种典型的多种金属离子的荧光螯合剂，从其水溶液中吸附引入到含表面活性剂的介孔中。在微孔中加入10 μl的15 μM Al^<3+>溶液，膜的荧光强度提高了约10倍。QS与Al^<3+>的络合在介孔附近成功发生。在QS存在的情况下，将1,10-菲罗啉（Phen）作为掩蔽剂引入介孔中，实现了Al^<3+>的选择性检测。15 μM的Mg^<2+>,Zn^<2+>, Cd^<2+>的干涉被Phen完全抑制，随后在干扰物质存在的情况下，只观察到Al^<3+>的荧光增强。此外，首次演示了使用由功能化mtf组成的传感器阵列对多样品进行同时分析。本研究证明的传感原理适用于对水溶液中多种化学物质的传感。此外，在水溶液中与分析物结合能力较差的受体，可能在含有表面活性剂的介孔中存在的疏水纳米尺寸的空腔中工作得很好。这项工作不仅有助于化学器件的构建，也有助于纳米化学的发展。

We have developed a novel chemical-sensing device based on the mesoporous thin films (MTFs) wherein both a fluoroionophore and a masking agent are embedded. Using this sensor, a highly selective detection of metal ions in an aqueous solution has been successfully demonstrated. The MTFs are deposited by casting the precursor solution onto the bottom of microwells on a silica microliter plate. The fluoroionophore 8-hydroxyquinoline-5-sulfonic acid (QS), which is a typical fluorogenic chelating agent for a variety of metal ions, is introduced into the mesopores containing surfactants by adsorption from its aqueous solution. The fluorescence intensity of the film is enhanced approximately 10 times by dropping 10 μl of 15 μM Al^<3+> solution onto the microwell. Complexation between QS and Al^<3+> occurs successfully in the vicinity of the mesopores. The selective detection of Al^<3+> is achieved by the introduction of 1,10-phenanthroline (Phen) as a masking agent into the mesopores in the presence of QS. The interferences of 15 μM of Mg^<2+>,Zn^<2+>, or Cd^<2+> are completely inhibited by Phen, and subsequently, fluorescence enhancement by only Al^<3+> is observed in the presence of the interfering species. Furthermore, simultaneous analyses of the multisamples using a sensor array composed of the functionalized MTFs are demonstrated for the first time.The sensing principle demonstrated in the present study is applicable to the sensing of a variety of chemical species in aqueous solutions. Furthermore, receptors that have poor binding capabilities to the analytes in aqueous solutions, probably work well within hydrophobic nanometer-sized cavities present in the mesopores containing surfactants. This work contributes not only to the construction of the chemical devices, but also to the development of nanochemistry.