Establishment of a rapid screening procedure of abused drugs and its forensic toxicological application

我们设计了一种快速筛选方法，并同时半定量分析了30种滥用药物，包括苯丙胺、苯丙胺、哌嗪、色胺和苯乙胺衍生的名牌药物和阿片类药物。尿样用尿素酶消化，经Focus、TM、Gt；柱固相萃取、乙酰化后，以扫描方式用气相色谱-质谱仪进行分析。使用保留时间锁定技术获得的保留时间和3个限定子离子用于获得阳性结果。根据所建立的方法，利用NAGINATA？&lt；TM&gt；定量化软件，根据各药物的保留时间、QT%、标准曲线的斜率和截距以及质谱图等参数，建立了“滥用药物”的数据库。我们考察了该数据库在法医毒理学案例中的应用，发现这项新技术简单，提供了关于样品中药物存在和数量的有用信息，而没有标准化合物。我们还建立了全血中13种苯丙胺相关药物、人全血和尿液中2种色胺以及人肝和肾中吗啡的可靠定量方法。这些方法被用于法医尸检案件的死因诊断。

We devised a rapid screening method for and simultaneous semi quantitative analysis of 30 abused drugs including amphetamines, amphetamine-, piperazine-, tryptamine-, and phenethylamine-derived designer drugs and opiates in human urine. The urine sample was digested with urease, and the drugs were analyzed by gas chromatography-mass spectrometry in the scan mode after solid phase extraction with Focus^<TM> column and acetylation. Retention time obtained with use of retention time locking technique and 3 qualifier ions were used to obtain positive results. Based on the established method, database of "abused drugs" was constructed from parameters in each drug including retention time, QT%, slope and intercept of calibration curve and mass spectrum using tentative quantification software NAGINATA^<TM>. We examined the usefulness of the database in forensic toxicological cases and found that this new technique was simple and gave useful information on the existence as well as amount of drugs in the sample without having standard compounds.We also established the reliable quantification procedures for 13 amphetamine-related drugs in whole blood,2 tryptamines in human whole blood and urine and morphine in human liver and kidney. The methods were used for diagnosing cause of death in forensic autopsy cases.