Precise cliagnosis and treatment of facial nerve palsy(Bell's palsy and Ramsay hunt syndrome)

关于治疗贝尔氏麻痹和拉姆齐·亨特综合征的类固醇最佳剂量的研究很少。在本研究中，我们进行了类固醇治疗1型单纯疱疹（HSV-1）面神经麻痹的剂量依赖性动物实验。此外，我们还研究了新型自由基清除剂依达拉奉对HSV-1型面神经麻痹的治疗作用。类固醇治疗面神经麻痹在临床、电生理和组织病理学上均有效。另一方面，根治性清除剂依达拉奉对面神经麻痹的恢复没有任何作用，反而增加了面神经麻痹的发生率。以HSV-1为载体，观察神经生长因子对面神经麻痹的影响。首先，将LacZ引入HSV-1基因组，并将病毒接种于小鼠耳廓。LacZ在颞内面神经中未见表达。然而，当HSV-1将LacZ接种到颞内面神经上时，LacZ在颞内面神经上被鉴定出来。然后，将HGF引入HSV-1，接种于颞内面神经。3周后，HGF的作用在电生理和组织病理学上得到证实。这些结果表明HSV-1可以作为神经生长因子的载体。

There is little study concerning optimum dosage of steroid for Bell's palsy and Ramsay Hunt syndrome. In the present study, we performed aninal study of dose dependent effect of steroid for facial nerve paralysis induced by herpes simplex type 1 (HSV-1). Moreover, we studied the effect of new radical scavenger of edaravone for facial nerve paralysis induced by HSV-1. Steroid was effective for facial nerve paralysis in both clinical, electrophysiological and histopathologically. On the other hand, radical scavenger of edaravone did not show any effect for the recovery of facial nerve paralysis but increased incidence of facial nerve paralysis.Using HSV-1 vector, effects of nerve growth factor for facial nerve paralysis was examined. At first, LacZ was introduced into HSV-1 genomes and the virus was inoculated onto auricle of mice. Expression of LacZ could not find in the intratemporal facial nerve. However, when HSV-1 introduced LacZ was inoculated directory onto the intratemporal facial nerve, LacZ was identified in the intratemporal facial nerve. Then, HGF was introduced into HSV-1 and inoculated it directory onto the intratemporal facial nerve. After 3 weeks, effect of HGF was confirmed in both electrophysiologically and histopathologically. These results suggest that HSV-1 can be useful as a vector for nerve growth factors.