Experimental studies on application of anti-adhesion therapy for retinal microcirculatory disturbances

本实验观察了白细胞与人视网膜血管内皮细胞的粘附。培养液中的高糖浓度和胰岛素增加了中性粒细胞与视网膜血管内皮细胞的粘附。内皮细胞的粘附分子之一细胞间粘附分子-1（ICAM-1）在高糖和胰岛素刺激下表达上调。抗ICAM-1抗体和蛋白激酶C抑制剂可抑制白细胞粘附。上述结果提示，葡萄糖和胰岛素均通过激活蛋白激酶C，增加视网膜血管内皮细胞ICAM-1的表达，而ICAM-1的表达增加是中性粒细胞粘附的原因。吖啶橙子荧光照相术用于研究激光治疗后视网膜中的白细胞动力学。白细胞增生的滚动 ...更多信息 不仅在光凝固区域而且在未处理的视网膜中观察到沿着视网膜静脉的TES沿着和增加的白细胞截留。OCT研究显示未治疗区域出现视网膜水肿。检测到视网膜和脉络膜中VEGF水平的时间增加。这些结果表明，通过散射光凝在视网膜和脉络膜中VEGF的暂时增加增强了视网膜白细胞停滞，这导致视网膜水肿的发展。我们还研究了Tenon’s下注射曲安奈德对大鼠视网膜散射光凝后增强的白细胞-内皮细胞相互作用和视网膜水肿的影响。结果表明，曲安奈德能有效地抑制白细胞在视网膜内的滚动和截留，改善视网膜水肿。这支持使用曲安奈德治疗黄斑水肿患者，以防止全视网膜激光光凝术后黄斑水肿恶化。少

Leukocyte adhesion to the human retinal vascular endothelial cells was studied in vitro. High glucose concentration and insulin in the culture medium increased the adhesion of neutrophils to the retinal vascular endothelial cells. One of the adhesion molecules of the endothelial cells, intercellular adhesion molecule-1 (ICAM-1), was up-regulated by the high glucose and insulin. The leukocyte adhesion was inhibited by anti-ICAM-1 antibody and the inhibitor of protein kinase C. These results suggested that both glucose and insulin increased the expression of ICAM-1 of the retinal vascular endothelial cells through the activation of protein kinase C and that the increased expression of ICAM-1 is the cause for the adhesion of netrophils.The effect of triamcinolone acetonide on the leukocyte adhesion after scatter retinal photocoagulation was studied in rats in vivo. Acridine orange fluorography was used to study leukocyte dynamics in the retina after laser treatment. The rolling of leukocy … More tes along the retinal veins and increased leukocyte entrapment were observed not only in the photocoagulated area but also in the untreated retina. The OCT study showed the development of retinal edema in the untreated area. The temporal increase in the level of VEGF in the retina and choroid was detected. These results suggest that temporal increase in the VEGF in the retina and choroid by scatter photocoagulation enhances retinal leukostasis which leads to development of retinal edema. We also studied the effect of sub-Tenon's injection of triamcinolone acetonide on the enhanced leukocyte-endothelial interaction and retinal edema after scatter retinal photocoagulation in rats. The results suggested that triamcinolne effectively inhibited the rolling and entrapment of leukocytes in the retina and improved the retinal edema after scatter photocoagulation. This supports the use of triamcinolone acetonide for patients with macular edema to prevent the worsening of macular edema after panretinal laser photocoagulation. Less